Picture from Bae YK et al. (2009) Curr Biol "The CIL-1 PI 5-phosphatase localizes TRP Polycystins to cilia and activates ...."

(D-E) Ppkd-2::CIL-1::tdTomato in male head (D) and tail (E). The tdtomato labels cilia (arrowheads), dendrites (den), axon, and small spots in cell bodies (arrows). (F) Intestinal expression of CIL-1 labels reticular structure in the cytoplasm (arrows). Scale bar, 10um.

Picture from Kaplan OI et al. (2012) Curr Biol "Endocytosis genes facilitate protein and membrane transport in C. elegans ...."

Colocalization of dpy-23 and mCherry-tagged CHE-13/IFT57 in multiple amphid or phasmid cilia. Arrows indicate pools of accumulated endocytosis-associated protein near the ciliary base. Arrowheads show pools of CHE-13::mCherry at the ciliary base. cil, cilia; den, dendrite. Scale bar represents 2 mm.

Picture from Kaplan OI et al. (2012) Curr Biol "Endocytosis genes facilitate protein and membrane transport in C. elegans ...."

Colocalization of clic-1 and mCherry-tagged CHE-13/IFT57 in multiple amphid or phasmid cilia. Arrows indicate pools of accumulated endocytosis-associated protein near the ciliary base. Arrowheads show pools of CHE-13::mCherry at the ciliary base. cil, cilia; den, dendrite. Scale bars represent 2 mm.

Picture from Kaplan OI et al. (2012) Curr Biol "Endocytosis genes facilitate protein and membrane transport in C. elegans ...."

Colocalization of rab-5 and mCherry-tagged CHE-13/IFT57 in multiple amphid or phasmid cilia. Arrows indicate pools of accumulated endocytosis-associated protein near the ciliary base. Arrowheads show pools of CHE-13::mCherry at the ciliary base. cil, cilia; den, dendrite. Scale bars represent 2 mm.

Picture from Blacque OE et al. (2006) Mol Biol Cell "The WD repeat-containing protein IFTA-1 is required for retrograde ...."

(A and B) GFP-tagged IFTA-1 localizes specifically at the base of cilia (transition zones or basal bodies) and along the ciliary axonemes of C. elegans ciliated neurons. Shown are fluorescence images of one set of phasmid neurons in the nematode tail (A), and one set of amphid cilia in nematode head (B), with the cell bodies (cb), dendrites (den), transition zones (tz), and ciliary axonemes (cil) indicated.

Picture from Bae YK et al. (2009) Curr Biol "The CIL-1 PI 5-phosphatase localizes TRP Polycystins to cilia and activates ...."

Figure S3. cil-1 expression and localization(A-C) The GFP expression driven by a 2.2kb bath-42 promoter (Pbath-42(2.2kb)::GFP) is broad in both hermaphrodites and males. In adult hermaphrodite head (A), adult male head (B), and tail (C), the 2.2kb bath-42 promoter is expressed in the pharynx (ph), intestine (in), andunidentified neurons (n). (D-E) Ppkd-2::CIL-1::tdTomato in male head (D) and tail (E). The tdtomato labels cilia (arrowheads), dendrites (den), axon, and small spots in cell bodies (arrows). (F) Intestinal expression of CIL-1 labels reticular structure in the cytoplasm (arrows). Scale bar, 10um.

Picture from Bell LR et al. (2006) Genetics "The molecular identities of the Caenorhabditis elegans intraflagellar ...."

Figure 6.-Expression of DYF-6TGFP in amphid neurons of dauer larvae. (A) Accumulation of fluorescence in the den- dritic endings of amphid neurons. An arrow marks the tran- sition zone. Fainter fluorescence can be seen in the dendritic bodies (arrowhead). (B) The corresponding Nomarski image of A. (C) Dorsal-ventral view of bright fluorescence in the cell bodies of amphid neurons in another dauer larva. Fainter fluorescence can also be seen in dendritic bodies (arrow- head). An arrow delimits autofluorescent gut granules just posterior to the terminal bulb of the pharynx. Both specimens had been in the dauer state for >3 days. Bars, 10 μm.

Picture from Kaplan OI et al. (2012) Curr Biol "Endocytosis genes facilitate protein and membrane transport in C. elegans ...."

Figure S1, Related to Figure 1 (A) Representative whole cell images of C. elegans ciliated sensory neurons showing the subcellular localisation of endocytic components. Shown are fluorescence images of head and tail regions of worms overexpressing the indicated GFP-tagged transgenes in amphid or phasmid neurons. Arrowheads indicate an enriched pool of protein found at the distal tips of dendrites, beneath the presumptive cilia, in a region we call the periciliary membrane compartment (PCMC). Transgenes expressed under gene promoters active in ciliated cells, namely che-12 (CLIC-1, CHE-13) [1], arl-13 (RAB-5) [2, 3], rab-3 (DPY-23) [4, 5] and str-1 (DYN-1b) [6]. Scale bars; 2 um (high magnification images) and 5 um (low magnification images). cil; cilia. den; dendrite.

Picture from Kaplan OI et al. (2012) Curr Biol "Endocytosis genes facilitate protein and membrane transport in C. elegans ...."

Figure S1, Related to Figure 1 (A) Representative whole cell images of C. elegans ciliated sensory neurons showing the subcellular localisation of endocytic components. Shown are fluorescence images of head and tail regions of worms overexpressing the indicated GFP-tagged transgenes in amphid or phasmid neurons. Arrowheads indicate an enriched pool of protein found at the distal tips of dendrites, beneath the presumptive cilia, in a region we call the periciliary membrane compartment (PCMC). Transgenes expressed under gene promoters active in ciliated cells, namely che-12 (CLIC-1, CHE-13) [1], arl-13 (RAB-5) [2, 3], rab-3 (DPY-23) [4, 5] and str-1 (DYN-1b) [6]. Scale bars; 2 um (high magnification images) and 5 um (low magnification images). cil; cilia. den; dendrite.

Picture from Kaplan OI et al. (2012) Curr Biol "Endocytosis genes facilitate protein and membrane transport in C. elegans ...."

Figure 1. Localizations of C. elegans Endocytosis-Associated Proteins Overlap with a Distinct Compartment at the Base of Sensory Cilia (A) Colocalization of indicated GFP-tagged endocytic proteins and mCherry-tagged CHE-13/IFT57 in multiple amphid or phasmid cilia. DYN-1 localization is shown in the AWB olfactory cilium. GFP-tagged fusion genes were expressed under gene promoters active in ciliated cells, namely che-12 (CLIC-1, CHE-13) [43], arl-13 (RAB-5) [29], rab-3 (DPY-23) [23], and str-1 (DYN-1b) [27]. The str-1p::dyn-1b::gfp and rab-3p::dpy-23::gfp constructs are functional (Figures 2D and 2E; Figure S2A; [23]); functional assessment using phenotypic rescue could not be determined for gfp::rab-5 and clic-1::gfp. Arrows indicate pools of accumulated endocytosis-associated protein near the ciliary base. Arrowheads show pools of CHE-13::mCherry at the ciliary base. cil, cilia; den, dendrite. Scale bars represent 2 mm. (B) Fluorescence intensity of CHE-13::mCherry relative to CLIC-1::GFP or GFP::RAB-5 indicating endocytosis-associated protein signals (green arrows) partly overlap with the IFT pool (red arrows). Data are shown from one experiment. White arrows indicate starting position of measurements shown below each panel. Graphs aligned with fluorescence images. a.u., arbitrary units. Scale bars represent 2 mm. (C) PHA/PHB neuronal cilia in WT animals expressing the transcriptional PHA/PHB marker, srb-6p::gfp. cil, cilia; TZ, transition zone; den, dendrite. Scale bar represents 1 um.