Picture from Lynch AM et al. (2012) Curr Biol "A genome-wide functional screen shows MAGI-1 is an L1CAM-dependent stabilizer ...."

Figure S1. MAGI-1 Localizes to a Unique Junctional Domain and Helps Regulate Enclosure, Related to Figure 3. Wild type embryos immunostained for HMP-2, MAGI-1, and AJM-1 (A), JAC-1::GFP and MAGI-1 (B), and MAGI-1 and AJM-1 (C). Junctions were examined using Volocity (A, Bi) and ImageJ (Ci). MAGI-1 does not primarily colocalize with the cadherin-catenin complex (CCC), or the DLG-1/AJM-1 complex (DAC), but rather resides between them, although there is some overlap between MAGI-1 and the CCC. A, B, C scale bar = 10m. Ai-iv, Bi, Ci scale bar = 0.5m. (D) Schematic of the C. elegans apical junction to include MAGI-1.

Picture from Ofulue EN et al. (1991) DNA Cell Biol "Molecular cloning and characterization of the Caenorhabditis elegans ...."

FIG. 4. Northern blot analysis. Total cellular RNA (20-25 ug) isolated from embryo (A) and various stages of nematode development (B), were denatured, separated on a 1% agarose-formaldehyde gel, and blotted onto a nylon membrane (Sambrook et al., 1989). After hybridization with the 761-bp PCR fragment (A), the 2.8-kb insert of the cDNA clone pCef6A (BI), or the 3-kb actin gene 1 insert of pCeA7 (BII), filters were washed as described in Materials and Methods and exposed to X-ray film for 5 hr (panel BI) or overnight at -70 C using Dupont Cronex intensifying screens. Lower panel. Ethidium bromide-stained gel prior to blotting. E, embryo; L1 to L4, larvae; D, dauer larva; A, young adult; M, size markers. Sizes of marker bands are given in kilobases.

Picture from Loer CM et al. (2015) Genetics "Cuticle integrity and biogenic amine synthesis in Caenorhabditis elegans ...."

(B) ptps-1-GFP reporters. Left: head showing NSM expression plus other neurons and nonneuronal cells, including anterior intestine (dorsal-ventral view, maximum intensity projection) (MIP); strain OH11619, described by Zhang et al. (2014). Right: vulval region showing expression in neurons VC4, 5 (weak) and HSN, plus lateral seam cells (lateral view, MIP); strain CZ18321.

Picture from Tamas I et al. (2005) BMC Genomics "A combined approach exploring gene function based on worm-human ...."

Figure 4. ZK795.3::GFP reporter expression. Evident in the excretory cell system (panel A) in about 20% of animals, but was always present in the excretory cell (labeled 'ex', panels A and Bi). The anal sphincter and/or depressor cell around the anus ('cl') also expressed GFP (panel Bii), as did the juvenile vulva ('v', panel Biii) and the spermathecae (panel C). Scale bar represents 10 um.

Picture from Tamas I et al. (2005) BMC Genomics "A combined approach exploring gene function based on worm-human ...."

Figure 2. F41D9.1::GFP expression. Widespread through the neural system. Panel A shows the general expression pattern in an L1 stage animal. Many cells in the nerve ring, the ventral nerve cord (vnc) and the tail region express GFP. Panel B presents the tail region in greater detail, scanning through the animal at three focal planes from right (Bi) to left (Biii), with the ventral side facing down. A cluster of laterally symmetrical cells is visible in panels Bi and Biii, whereas in Bii cells of the vnc are visible. Magnification is 100. Fig C presents 3 focal planes from dorsal (Panel Ci) to ventral (Panel Ciii) through the worm head, with the posterior pharyngeal bulb to the left. The GFP images have undergone deconvolution to increase resolution. Cells of the dorsal ganglion are visible in Ci, the retrovesicular ganglion is marked in Cii, and processes leading to it are indicated in Ciii. Scale bar represents 10 um.

Picture from Ofulue EN et al. (1992) DNA Cell Biol "Isolation and characterization of eft-1, an elongation factor 2-like gene on ...."

FIG. 6. Northern blot analysis. A. 5 ug poly(A)+RNA (lane 1) and 20 ug total cellular RNA (lane 3) from embryos. B. 25 ug total RNA (rows I) from all stages of development were hybridized with nick-translated 32P-labeled B3255 (panels A and BI) or with labeled pCeA7 (C. elegans actin gene 1, panel BII). E, embryo; L1-L4, larvae; D, dauer larva; A, young adult; M, size markers. Sizes of marker bands are given in kilobases.

Picture from Ho J et al. (2018) MicroPubl Biol "Characterizing Dyneins Role in P-cell Nuclear Migration using an Auxin-Induced ...."

Figure 1. Auxin-induced degradation of dynein causes a P-cell nuclear migration defect. Average number of GABA neurons in L4 animals that express the TIR1 gene (an auxin-induced E3 ubiquitin ligase; Zhang et al., 2015) specifically in P cells. Animals either expressed the DHC-1 degron tag (UD551) or did not express the DHC-1 degron tag (UD550). These animals (UD551 and UD550) were either exposed to auxin (+) or not (-) as L1 animals. Statistical significance calculated by t-test with p-value<0.0001. The mean is marked with error bars denoting 95% CI.

Picture from Li C et al. (2008) PLoS Genet "An essential role for DYF-11/MIP-T3 in assembling functional intraflagellar ...."

DYF-11 is a component of the intraflagellar (IFT) transport machinery. Kymograph analyses show the bi-phasic anterograde movement of DYF-11::GFP in amphid cilia. Middle segment (MS) motion was determined to be 0.74±0.08 μm/sec, while the distal segment (DS) velocity was 1.15±0.21 μm/sec. The second and fourth panels are actual kymographs, whereas the third and fifth panels are representative traces of moving particles in the indicated middle or distal segments; X axes denote time whereas Y axes denote distance covered by fluorescently-labeled particles. In the still fluorescence microscopy image taken from Movie S1 (left panel), solid arrowheads denote the positions of representative transition zones, while hollow arrowheads denote unexpected dendritic endings. Arrowheads represent the positions of transition zones and brackets show ciliary axonemes. Scale bar, 5 μm

Picture from Maurer CW et al. (2007) Development "Timing of the onset of a developmental cell death is controlled by ...."

Expression of ced-3 promoter::GFP transgenes is induced shortly before tail-spike cell death. (A) Cartoon showing the tail-spike cell at different stages of development. Filled green ovals, tail-spike cell nuclei; gray oval, dying tail-spike cell corpse. Ball-stage embryo (left); threefold-stage embryo (center and right). (B-D) Merged DIC and GFP images of threefold-stage embryos expressing a 1.5 kb C. elegans ced-3 promoter::GFP reporter transgene (nsEx723). (B) Embryo before the onset of tail-spike cell GFP expression (arrow and inset: binucleate tail-spike cell). (C) Embryo at onset of tail-spike cell GFP expression (arrow and inset: bi-nucleate tail-spike cell). (D) Embryo approximately 31 minutes after the onset of tail-spike cell GFP expression (arrow and inset: dying tail-spike cell corpse). Scale bars: 10 μm.

Picture from Maurer CW et al. (2007) Development "Timing of the onset of a developmental cell death is controlled by ...."

Figure 1. Expression of ced-3 promoter::GFP transgenes is induced shortly before tail-spike cell death. (A) Cartoon showing the tail-spike cell at different stages of development. Filled green ovals, tail-spike cell nuclei; gray oval, dying tail-spike cell corpse. Ball-stage embryo (left); threefold-stage embryo (center and right). (B-D) Merged DIC and GFP images of threefold-stage embryos expressing a 1.5 kb C. elegans ced-3 promoter::GFP reporter transgene (nsEx723). (B) Embryo before the onset of tail-spike cell GFP expression (arrow and inset: binucleate tail-spike cell). (C) Embryo at onset of tail-spike cell GFP expression (arrow and inset: bi-nucleate tail-spike cell). (D) Embryo approximately 31 minutes after the onset of tail-spike cell GFP expression (arrow and inset: dying tail-spike cell corpse). (E) Merged DIC and GFP image of a threefold-stage embryo expressing an egl-1 promoter::GFP integrated transgene (bcIs1). Arrow and inset: tail-spike cell corpse lacking GFP. Scale bars: 10 μm.