Figure 3. REI-1 Is Expressed in the Germline and Co-localizes withRAB-11.1 on Late-Golgi Membranes(A) Schematic diagram of the C. elegans gonad. Orange indicates the egg shell.(B and C) Expression of REI-1 in the germline. Transgenic animals expressing GFP-REI-1 under the control of the
rei-1 promoter were observed by confocal microscopy. Images of the germline (B) and oocyte (C) were shown.(D) Image of the CG marker CAV-1-GFP in an oocyte. Magnified images of GFP-REI-1 and CAV-1-GFP on the CGs are also shown in insets (C and D). (E-G) GFP-REI-1 (E, green in G) co-localizes with mCherry-RAB-11.1 (F, magenta in G) on punctate structures in growing oocytes.(H-S) Subcellular localization of REI-1 in embryos. (H-J) GFP-REI-1 (H, green in J) co-localizes with the late-Golgi marker mCherry-SYN-16 (I, magenta in J).(K-M) GFP-REI-1-positive puncta (K, green in M) are juxtaposed to mCherryRER-1-positive early-Golgi membranes (L, magenta in M). (N-P) GFP-REI-1 (N, green in P) partly co-localizes with mCherry-RAB-11.1 (O, magenta in P).(Q-S) Part of GFP-RAB-11.1 (Q, green in S) localizes to the mCherry-SYN-16-positive late-Golgi membranes (R, magenta in S).The regions surrounded by squares are magnified in the insets (C-S). GFPRAB-11.1-positive puncta without overlapping mCherry-SYN-16 signals are also present (Q-S, dotted squares). SP, spermatheca. Scale bars, 10 um.