Figure 1. Analysis of temperature-sensitivity in orthologous mutations:(A) Graph showing sterility of C. briggsae mutant hermaphrodites at three temperatures. Circles represent the percent displaying the trait, and lines represent 95% confidence limits, calculated using the Wilson/Brown method, implemented by GraphPad Prism. (B) Differential Interference Contrast (DIC) image of a
glp-4(
v473) XX animal at 25° C. The yellow arrow marks the vulva. There is no visible germ line. Anterior is left and ventral is down. (C) DIC image of a
fog-1(
v441) XX animal at 20°C. The yellow arrow marks the vulva. The uterus is empty and only oocytes are differentiating in the germ line. Three of them are outlined in yellow. (D) DIC image of a heterozygous
fog-1 XO male, showing oocytes outlined in yellow, as well as sperm (white arrows).