Picture from Du H-P et al. (1996) Neuron "Extracellular proteins needed for C. elegans mechanosensation."

Figure 8. Beta-Galactosidase Expression from mec-9-lacZ Fusions. All animals are L4 larvae.(a) Long transcript expression in the touch receptor neurons from TU#165.(b) Short transcript expression from TU#160 in some ventral cord motor neurons and cells in the head.(c) Expression from both transcripts using TU#171. Both sets of cells stain.

Picture from Du H-P et al. (1996) Neuron "Extracellular proteins needed for C. elegans mechanosensation."

Figure 4. Beta-Galactosidase Expression from a mec-5-lacZ Fusion (TU#235). (A) Newly hatched larva.(B) L2 stage larva.

Picture from Wang H et al. (2013) Curr Biol "Neuropeptide secreted from a pacemaker activates neurons to control a rhythmic ...."

(C) The expression pattern of snt-2. Representative fluorescent image of an adult expressing a snt-2 transcriptional reporter, in which GFP is driven by the snt-2 promoter fragment (4,177 bp, from 792 bp upstream of ATG to 33 bp of the second exon of snt-2 gene). Arrowheads indicate fluorescence in head and tail.

Picture from Agostoni E et al. (1999) Gene "Identification and characterization of a new member of the gas3/PMP22 gene ...."

Fig. 5. Developmental Northern blot analysis of C01C10.1b and C01C10.1a. The C01C10.1b transcript, at about 1150 bp, and the 950-bp transcript of C01C10.1a are present in all developmental stages. The C. elegans initiation factor was used to visualize the relative amount of loaded mRNA.

Picture from Fay, Samuel F. et al. MicroPubl Biol

Figure 1. Summary of CRISPRcruncher: (A) Example CRISPRcruncher output displaying options for introducing restriction sites (>6 bp) within the first 33 coding nucleotides of CELE_F54B11.2. For simplicity, only 17/65 lines of output are shown. Red letters indicate changes from the input sequence; bold letters indicate the newly introduced endonuclease recognition site. 'Strand' column indicates whether the top (shown) or bottom strand contains the recognition motif; both indicates that the motif is an inverted repeat (a.k.a. palindrome). (B-G) Analyses of site frequencies for five randomly selected genes on the X chromosome. The first 99 coding nucleotides within the first exon of each gene were examined either as a contiguous sequence (B,C) or as three nonoverlapping 33-bp segments (D-G). 'Total Reported REs' indicates the complete set of REs, including isoschizomers and other categories of related motifs, capable of cleaving at the identified new sites. 'Distinct Sequences' indicates the total number of distinct new 33-bp or 99-bp sequences identified by CRISPRcruncher. Parameters were set to identify new restriction sites of 4 bp or greater (>4 cutter; red circles) or 6 bp or greater (>6 cutter; blue circles). (H) Percent of palindromic versus non-palindromic restriction motifs for the five 99-bp sequences. For supporting information, see Extended Data.

Picture from Zheng Q et al. (2002) J Biol Chem "Molecular cloning and characterization of a novel alpha ...."

Figure 6. The relative RT-PCR of CE2FT-1 mRNA from C. elegans at different stages. A, total C. elegans RNA from the populations of L1, L2-L4, and adult stage were prepared and used as templates in first strand cDNA synthesis. A 500-bp positive control from the RT-PCR kit was used as a control for the RT-PCR method (Line Positive Control). The arrows indicate the expected size of alpha-actin and CE2FT-1. Size markers in bp are indicated. B, the quantitative real-time RT-PCR of CE2FT-1 mRNA from C. elegans at different stages. Total C. elegans RNA from the populations of L1, L2-L4, and adult stage were prepared and used as templates in first strand cDNA synthesis. A 106-bp cDNA was amplified. The expression level was indicated by threshold cycles. C, confirmation of a single product of amplification in quantitative real time RT-PCR was performed on 1.2% agarose gel. A 106-bp cDNA fragment was amplified from CE2FT-1, and a 100-bp cDNA fragment was amplified from actin. Size markers in bp are indicated.

Picture from Zhang Y et al. (2002) Mech Dev "MTD-1, a touch-cell-specific membrane protein with a subtle effect on touch ...."

Fig. 4. Expression of mtd-1::gfp (TU#677) in (A) an embryo, (B) a L3 wild-type larva, (C) a L2 wild-type larva, and (D) a L3 wild-type larva. Touch cells are indicated by arrows. (E) Effect of a synthetic transmembrane domain (red) on the expression of mtd-1::lacZ and mtd-1::gfp fusions.

Picture from Barrios L et al. (1989) J Mol Biol "Genomic organization of the glyceraldehyde-3-phosphate dehydrogenase gene ...."

Figure 8. Ontogeny of mRNAs for gpd-4 and gpd-2. 10 g of total embryonic (E) and postembryonic larval (L4) mRNAs were dot blotted on to nitrocellulose paper and probed with gpd-2 and gpd-4-specific probes. The probe for gpd-2 was prepared using pSS1.3 (Fig. 3) derived from an M13 phage DNA using an oligonucleotide primer located 196 bp from its termination codon, which extended to a HaeIII site 5 bp before its stop codon. Since the 5' end of gpd-3 obtains a trans-spliced leader sequence (see Results and Discussion) this probe is specific for gpd-2 only. The gene-specific probe for gpd-4 was prepared using pB9 (Fig. 1(b)), an oligonucleotide 164 bp downstream from its termination codon, which was then extended to a HaeIII site located 10 bp beyond its stop codon.

Picture from Jiu Y et al. (2012) PLoS One "Exocyst subunits Exo70 and Exo84 cooperate with small GTPases to regulate ...."

Figure S1 Confocal image of adult hermaphrodites expressing Psec-6::GFP under the 2065 bp promoter (Ex[Psec-6::GFP; pRF4]). Left is anterior. Scale bar, 100 um.

Picture from Jiu Y et al. (2012) PLoS One "Exocyst subunits Exo70 and Exo84 cooperate with small GTPases to regulate ...."

Figure 1. Expression patterns of exocyst subunit exoc-7 in C. elegans. Confocal images of adult hermaphrodites expressing Pexoc-7::GFP under the 2954 bp promoter (Ex[Pexoc-7::GFP; pRF4]).