Figure 1. pals gene responses to Orsay virus infection in young and aged animals: (A) Schematic of the reporter assay. ERT711
rde-1(
ne219) V; jyIs8[
pals-5p::GFP +
myo-2p::mCherry] X were synchronized and inoculated with liquid Orsay virus or no-virus control at young (day 1 of adulthood) and aged (day 5 of adulthood) stages. The animals were then scored for fluorescence according to a qualitative scale. Scale: 0.1 mm. Virus image from (Tao & Guo, 2014). (B) Quantification of the reporter expression in young and aged ERT711 upon Orsay virus infection. Animals were infected with equal concentrations of the virus and scored as indicated in (A). Statistical analysis was performed using chi-squared test with continuity correction. "***", p<0.001. (C) pals gene mRNA expressions in young and aged animals 24 hours post-inoculation with equal virus concentrations. mRNA expressions were quantified using qPCR in three biological replicates and compared using Student's t-test. "**", p<0.01; "***", p<0.001. (D) Viral RNA levels in young and aged animals 24 hours post-inoculation with equal virus concentrations. Viral RNAs were quantified using qPCR in three biological replicates and compared using Student's t-test. "ns", p>0.05; "**", p<0.01. (E) Viral RNA2 levels in young animals inoculated with various concentrations of liquid Orsay virus. Viral RNAs were quantified using qPCR in three biological replicates. Statistical analysis was performed by comparing the samples against aged animals inoculated with 100% liquid Orsay virus using One-way ANOVA test. "ns", p>0.05. (F) pals gene mRNA expressions in young and aged animals 24 hours post-inoculation with equal viral loads. Young and aged animals were inoculated with 5% and 100% Orsay virus, respectively. mRNA expressions were quantified using qPCR in three biological replicates and compared using Student's t-test. "ns", p>0.05.