Picture from Sun Y et al. (2013) PLoS One "The F-box protein MEC-15 (FBXW9) promotes synaptic transmission in GABAergic ...."

Figure 1. Behavioral defects in mec-15 mutants and expression of mec-15. (A) mec-15 mutants paralyze faster than wild-type control animals in the aldicarb assay. Paralysis of 20 animals of the indicated genotypes was scored over 2 hours. Fast paralysis of mec-15 mutants is rescued by expressing mec-15 specifically in GABAergic neurons (rescue). Data are averages from 3 independent experiments +/2 standard error of the mean (SEM). (B) Representative fluorescence images of young adults expressing GFP under the control of the mec-15 promoter (Pmec-15::GFP, top panel) and mCherry in GABAergic motor neurons (Punc-25::mCherry, middle panel). Co-expression of GFP and mCherry indicates expression of mec-15 in GABAergic motor neurons (cell bodies are labeled with white arrowheads in the merged image). Expression of GFP alone indicates expression of mec- 15 in cholinergic motor neurons (three such cell bodies are labeled with blue arrows). Scale bar is 20 um.

Picture from Sun Y et al. (2013) PLoS One "The F-box protein MEC-15 (FBXW9) promotes synaptic transmission in GABAergic ...."

(B) Representative fluorescence images of young adults expressing GFP under the control of the mec-15 promoter (Pmec-15::GFP, top panel) and mCherry in GABAergic motor neurons (Punc-25::mCherry, middle panel). Co-expression of GFP and mCherry indicates expression of mec-15 in GABAergic motor neurons (cell bodies are labeled with white arrowheads in the merged image). Expression of GFP alone indicates expression of mec- 15 in cholinergic motor neurons (three such cell bodies are labeled with blue arrows). Scale bar is 20 um.

Picture from Sun Y et al. (2024) MicroPubl Biol "Loss of function in rpms-1 does not enhance phenotypes of rpm-1 ...."

Figure 1. Double mutants of rpm-1 rpms-1 resemble rpm-1 single mutants: A. Schematics of rpm-1 and rpms-1 gene model. Black boxes represent exons. Blue arrowheads point at gRNA targeting sites for CRISPR-Cas9 genome editing. The deletion alleles ok364 and ju1285 result in truncated proteins due to frameshift followed by premature stop codons.B. Quantification of axon termination defects and absence of synapse branch of PLM neuron visualized by muIs32(mec-7p::GFP). Numbers of animals analyzed are shown below the bar graphs. Statistics: one-way ANOVA test for multiple comparison corrected with the false discovery rate (FDR) method of Benjamini and Hochberg. Error bars: SEM. ****p<0.0001, *0.01<p<0.05, ns=p>0.05.C. Quantification of the total number of synaptic puncta of GABAergic neurons in the dorsal nerve cord (DNC) visualized by juIs1(unc-25p::SNB-1::GFP). Numbers of animals analyzed are shown in the bar graphs. Statistics: unpaired t test. Error bars: SEM. ns=p>0.05.D. Representative images of knock in GFP::DLK-1(ju1579) in the nerve ring region. White dashes outline the region of interest (ROI) for quantification in E. Scale bar: 20 µm.E. Quantification of the fluorescence intensity of GFP::DLK-1(ju1579) normalized to the average value of wildtype (wt) animals. Numbers of animals analyzed are shown below the bar graphs. Statistics: one-way ANOVA test for multiple comparison corrected with the false discovery rate (FDR) method of Benjamini and Hochberg. Error bars: SEM. ****p<0.0001, ns=p>0.05.F. Quantification of PLM axon regrowth length after laser axotomy, normalized to the average value of wildtype (wt) animals. Numbers of animals analyzed are shown below the bar graphs. Statistics: one-way ANOVA test for multiple comparison corrected with the false discovery rate (FDR) method of Benjamini and Hochberg. Error bars: SEM. ns=p>0.05.

Picture from Penkner AM et al. (2009) Cell "Meiotic chromosome homology search involves modifications of the nuclear ...."

Figure 1. SUN-1 Forms Aggregates at the Nuclear Envelope during the Time of Ongoing Homolog Pairing (A) A mtf-1/sun-1::gfp;mtf-1/sun-1(ok1282) hermaphrodite gonad expressing SUN-1::GFP (middle, green in merge) was stained for DNA with DAPI (top, blue in merge). (B) SUN-1 staining (iii, red in merge) of hermaphrodite gonads expressing ZYG-12ABC::GFP (ii, green in merge). i, DAPI; iv, merge. The yellow arrowhead marks the position of a patch, while the yellow arrow highlights a focus. (C) DAPI staining (i, iii) and antibody staining (ii, iv). PC-binding proteins HIM-8 or ZIM-3 (red) are shown in combination with SUN-1::GFP (green). The green channel is shifted to the right to allow better visualization of green and red signals. (D) Centrosome marked by SPD-5 staining (red) in mitotic (i) and meiotic (ii, transition zone) nuclei expressing SUN-1:: GFP (green). Scale bars represent 10 um. (E) Western blot to show the in vivo interaction of ZYG12ABC::GFPand endogenous SUN-1 (54 kD, red arrowheads). Coimmunoprecipitations were performed with antiGFP antibodies. SUN-1 was detected with anti-SUN-1 antibodies. Genotypes are on the right.

Picture from Penkner AM et al. (2009) Cell "Meiotic chromosome homology search involves modifications of the nuclear ...."

Figure 1. SUN-1 Forms Aggregates at the Nuclear Envelope during the Time of Ongoing Homolog Pairing (A) A mtf-1/sun-1::gfp;mtf-1/sun-1(ok1282) hermaphrodite gonad expressing SUN-1::GFP (middle, green in merge) was stained for DNA with DAPI (top, blue in merge). (B) SUN-1 staining (iii, red in merge) of hermaphrodite gonads expressing ZYG-12ABC::GFP (ii, green in merge). i, DAPI; iv, merge. The yellow arrowhead marks the position of a patch, while the yellow arrow highlights a focus. (C) DAPI staining (i, iii) and antibody staining (ii, iv). PC-binding proteins HIM-8 or ZIM-3 (red) are shown in combination with SUN-1::GFP (green). The green channel is shifted to the right to allow better visualization of green and red signals. (D) Centrosome marked by SPD-5 staining (red) in mitotic (i) and meiotic (ii, transition zone) nuclei expressing SUN-1:: GFP (green). Scale bars represent 10 um. (E) Western blot to show the in vivo interaction of ZYG12ABC::GFPand endogenous SUN-1 (54 kD, red arrowheads). Coimmunoprecipitations were performed with antiGFP antibodies. SUN-1 was detected with anti-SUN-1 antibodies. Genotypes are on the right.

Picture from Jarriault S et al. (2008) Proc Natl Acad Sci U S A "A Caenorhabditis elegans model for epithelial-neuronal ...."

(A) The epithelial marker CHE-14::GFP is expressed in Y. (Right) Fluorescent photomicrograph of Y expressing CHE-14::GFP in a newly hatched L1 animal (40 of 40 early L1s expressed che-14 in Y). (Left) The corresponding Nomarski photomicrograph. CHE-14 can be detected at the apical side of the Y cell, bordering the rectal slit (white arrow), and some faint fluorescence can be seen in Y cytoplasm; note that both sides of the rectal slit are marked with CHE-14, although only one is shown in focus; arrowhead, Y nucleus.

Picture from Reichman R et al. (2018) Genetics "Mitotic and Meiotic Functions for the SUMOylation Pathway in ...."

D) 3XFLAG::UBC-9 and SUN-1 colocalize the germline.3XFLAG::UBC-9 and SUN-1 images were taken in the pre-meiotic tip, transition zone, andmid/late pachytene. Colocalization is enhanced in the TZ and MLP.

Picture from Jarriault S et al. (2008) Proc Natl Acad Sci U S A "A Caenorhabditis elegans model for epithelial-neuronal ...."

Fig. 4. Marker expression in Y and PDA. (A) The epithelial marker CHE-14::GFP is expressed in Y. (Right) Fluorescent photomicrograph of Y expressing CHE-14::GFP in a newly hatched L1 animal (40 of 40 early L1s expressed che-14 in Y). (Left) The corresponding Nomarski photomicrograph. CHE-14 can be detected at the apical side of the Y cell, bordering the rectal slit (white arrow), and some faint fluorescence can be seen in Y cytoplasm; note that both sides of the rectal slit are marked with CHE-14, although only one is shown in focus; arrowhead, Y nucleus. (B) The epithelial markers DLG-1 and AJM-1 are expressed in Y. Fluorescent photomicrographs of the subapical part of the Y cell (white arrowheads) expressing both DLG-1 (green, Left) and AJM-1 (red, Center) in the same L1 animal (overlay in yellow, Right; 41 of 41 and 27 of 27 early L1s expressed dlg-1 or ajm-1 in Y respectively); thin arrow, rectal slit. (C) PDA is a neuron in the L3 stage. Photomicrograph of the PDA motor neuron cell body (arrowhead) and axonal process (thick arrow) in a cog-1::gfp L3 larva. PDA's axon is first directed toward the posterior of the animal on its ventral side, then it runs round to the dorsal side through a right-handed commissure past the rectum (thick arrow) and goes toward the anterior part of the worm in the dorsal cord. In cog-1::gfp or ace-3/4::gfp animals, PDA's process (thick arrow) stops just posterior to the pharynx (asterisk). The anus is indicated by a thin arrow.

Picture from Minn I et al. (2009) Mol Biol Cell "SUN-1 and ZYG-12, mediators of centrosome-nucleus attachment, are a functional ...."

Figure 2. C termini of ZYG-12 and SUN-1 are oriented toward thelumen of the nuclear envelope. (A) Diagrams of SUN-1 and ZYG-12.TM, transmembrane domain; CC, coiled-coil domain; K, KASHdomain. Black bar indicates C-terminal 242-amino acid fragment ofSUN-1 and N-terminal 236-amino acid fragment of ZYG-12 used forraising the -SUN-1 and -ZYG-12 polyclonal antibodies, respectively.Numbers represent the amino acid positions of predicteddomains. (B) Fluorescence protease protection assay using syncytialgonads from animals expressing YFP::LMN-1 (qaIs3502[unc-119() pie-1::YFP::LMN-1 pie-1::CFP::H2B]). Immunostaining was performedusing -GFP mAb 3E6 (Invitrogen) to detect YFP::LMN-1and -SUN-1 antibody (Fig. 2 A) to detect SUN-1. Signals fromLMN-1 after trypsin treatment were captured using 30-fold longerexposure relative to those without trypsin treatment. Bar, 10 um. (C)Fluorescence protease protection assay using syncytial gonads fromwild-type animals. Signals from ZYG-12 after trypsin treatmentwere captured using 20-fold longer exposure relative to those withouttrypsin. Bar, 10 um.

Picture from Roelens, Baptiste et al. (2022) MicroPubl Biol

Figure 1. Localization of HIM-19:3xFLAG in the C elegans germline:A) Co-immunodetection of HIM-19::3xFLAG and nuclear envelope protein SUN-1 phosphorylated on serine 24 (SUN-1 S24-Pi), a marker of nuclei with active CHK-2, in a whole-mount gonad from phenotypically wild-type worm expressing 3xFLAG-tagged HIM-19 from the endogenous him-19 locus. White arrowheads indicate "outlier" nuclei with persistent CHK-2 activity; scale bar represents 20µm. B) Zoomed-in images of nuclei from the regions indicated by white boxes in A. Orange arrowheads indicate nuclei in which bright HIM-19::3xFLAG signals coincide with sites of SUN-1 S24-Pi enrichment; scale bars represent 2µm. Images in A and B are maximum intensity projections through the top layer of nuclei of the gonad, except for the mitotic zone image in B, in which a single equatorial slice is depicted to highlight the localization of HIM-19::3xFLAG at the nuclear periphery.