Picture from Katz DJ et al. (2009) Cell "A C. elegans LSD1 demethylase contributes to germline immortality by ...."

(D-H) Immunofluorescence with (D and G) SPR-5 N and (E and F) C-terminal Abs showing (D) the adult gonad, (E) oocytes, (F) an early embryo, (G) sperm, and (H) the corresponding DAPI image of sperm. The insets in (E), (F), and (G) are zoomed in on part of the panels. The identical staining pattern was observed with both N- and C-terminal Abs.

Picture from Moses D et al. (2025) MicroPubl Biol "Quiescent behavior in response to bacterial infection in C. ...."

Figure 1. Locomotion quiescence in C. elegans following infection by virulent S. marcesens: A. Minutes of quiescence in the first hour following 48 hours of exposure to S. marcesens pigmented (S. mar P) (n=12), S. marcescens non-pigmented (S. mar NP) (n=12), mixed S. marcesens pigmented and E. coli (n=12), or E. coli (n=4) *p<.05, **p<.01, based on Dunn's multiple comparisons test. B. Percentage of total worms removed from the edge of the plate at 30 and 60 minutes in a motivated displacement assay after 48 hours of exposure on S. marcesens pigmented (n=95), S. marcescens non-pigmented (n=126), or E. coli (n=102) (data points show mean of three trials with standard deviation) *p<.05 based on Tukey's multiple comparisons test. C. Survival assay showing living worms counted each day on each strain of S. marcesens and E. coli for six days (n=50 for each group) **p<.01, ***p<.001, ****p<.0001, based on log-rank (Mantel-Cox) test. D. Relative quiescence of mutant strains as compared to wild-type in the first hour following 48 hours of exposure to S. marcesens pigmented. All mutants are normalized to wild-type controls measured on the same WorMotel. No significant difference between wild-type and mutant C. elegans based on Dunn's multiple comparisons test (n=16 per group).

Picture from Amin NM et al. (2010) Development "The FoxF/FoxC factor LET-381 directly regulates both cell fate specification ...."

(B-D) Representative images of live embryos (B,C) and fixed late L1 larva (D) expressing let-381::gfp. (E-N) The left side of three L1 larvae double-labeled with LET-381::GFP (E,I), anti-FOZI-1 antibody (F,J) and DAPI (H) at the 14-M (E-H) and 16-M stage (I-K). (G,K) Merged images of E and F and of I and J, respectively. (L-N) An L1 larva double-labeled with anti-LET-381 (L) and anti-FOZI-1 antibodies (M) at the 16-M stage. (N) A merged image of L and M. (O) Summary of LET-381 expression in the M lineage, with LET-381-positive cells in green circles and CEH-34-positive cells outlined in black.

Picture from Au C et al. (2009) PLoS One "SMF-1, SMF-2 and SMF-3 DMT1 orthologues regulate and are regulated ...."

SMF-2::GFP is seen in cytoplasmic organelles in mc and vpi cells (E, cell plasma membranes are marked by a dotted line, N indicate the position of the nuclei). Scale bars are 5 um.

Picture from Shim J et al. (2004) Mol Biol Cell "The unfolded protein response regulates glutamate receptor export from the ...."

(A and B) Wild-type nematodes contain GLR-1::GFP in punctate structures in the cell body and neurites (100%, n = 42).(E-H) GLR-1::GFP (green) costained with DAPI (red) showing the proximal neurites of the nerve ring (bracket). (I-L) Endogenous GLR-1 detected by immunofluorescence (green) and costained with DAPI (red). (E and I) Wild-type nematodes contain GLR-1 in the proximal neurites of the nerve ring (bracket, 100%, n = 46) and in punctate structures in the cell body (arrowheads; 76%, n = 46). (L) No GLR-1 immunofluorescence is detected in the cell bodies of glr-1 null mutants (100%, n = 30), and (H) no GLR-1::GFP is detected in the cell bodies of nematodes lacking the transgene. Nematodes that express GLR-1::CFP (M) and the Golgi marker MANS::YFP (N) show colocalization at puncta in the cell body (O, merge, 100%, n = 22).

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(E, E') Pgrl-9::GFP is expressed in muscle or marginal cells in the terminal bulb of the pharynx.

Picture from Klimas AS et al. (2023) MicroPubl Biol "The C. elegans deglutamylase CCPP-6 does not operate redundantly with ...."

Figure 1. ccpp-1; ccpp-6 double mutant phenotypes are not indicative of redundancy:A) Viability of the single and double mutants is comparable to WT. Dye-filling of amphid (B-E) and phasmid (F-H) neurons at L1 (B), L2/3 (~40h post laying; C&F), L4 (D&G), and adult (E&H) life stages. Three (L4 and adult) or four (L1 and L2/3) independent trials were conducted, each trial had an n of >15 worms/genotype (total n: L1 >120; L2/3 >97; L4 >55; adult >63).

Picture from Dozier J et al. (2024) MicroPubl Biol "Sterility in the offspring of spr-5; met-2 mutants may be caused by ...."

Figure 1. Knocking down SET-2 and MES-4 partially rescues the germline in spr-5; met-2 progeny: 40x Differential Interference Contrast (DIC) (A, C, E G) and DAPI (B, D, F, H) images of wildtype (N2 strain) (A-B), or spr-5; met-2 (C-H) progeny from hermaphrodite parents fed control (empty vector L4440) RNAi (A-D), set-2 RNAi (E-F), or mes-4 RNAi (G-H) three days (wildtype fed control RNAi) (A-B), four days (spr-5; met-2 fed set-2 or mes-4 RNAi) (E-H), and five days (spr-5; met-2 fed control RNAi) post hatching (C-D) when progeny reached young adulthood. Dashed lines outline the posterior gonad arm (A-B, E-H) or the entire germline (C-D). Asterisks (*) denote oocytes. Arrow denotes an embryo. Scale Bar: 20µm. (I) Quantification of the total number of oocytes counted across both gonad arms from wildtype progeny on control RNAi (N=48) and spr-5; met-2 progeny on either control (N=50), set-2 RNAi (N=50), or mes-4 RNAi (N=50) (unpaired t-test, **** represent a p-value <0.0001, *** represent a p-value <0.05).

Picture from Alexander M et al. (2009) Development "An UNC-40 pathway directs postsynaptic membrane extension in Caenorhabditis ...."

(E-E'') UNC-73B::CFP specifically expressed in muscles in the background of the trIs34 integrated transgene that expresses UNC-40::YFP specifically in muscles. The localization of fusion proteins to the muscle arm termini is indicated with a yellow arrowhead. Scale bars: 50 μm in A-C; 10 μm in E-E''.

Picture from Bowles, Adria K et al. (2019) MicroPubl Biol

Figure 1 fem-3(e2006) L4 hermaphrodites were placed on lawns of OP50 E. coli (n=37), wildtype (wt.) V. vulnificus (n=65), or tonB1 tonB2 double mutant V. vulnificus (n=71) and assessed every 24 hrs. for survival. A Log-rank test indicated that there was no significant difference between survival on the two V. vulnificus strains (p=0.6), while both V. vulnificus strains cause significant reductions in survival relative to growth on OP50 (p<0.001).