BIR-1 localizes to subchromosomal foci on meiotic chromosomes. Antibody staining is presented in green and DAPI staining in red. (M-P) Only merged images are shown for BIR-1 at corresponding stages. Arrows in M-N point to the staining between homologues in meiosis P, they point to the staining between sister chromatids in meiosis II. Bar, 2 um.
AIR-2 localizes to subchromosomal foci on meiotic chromosomes. Antibody staining is presented in green and DAPI staining in red. (A-L) Representative slice images of AIR-2 (A, D, G, and J), DAPI (B, E, H, and K), or merged (C, F, I, and L) staining in oocyte-derived nuclei at different meiotic stages. Arrows in A-F point to the staining between homologues in meiosis I, and in J-L, they point to the staining between sister chromatids in meiosis II.
Figure 3. AIR-2 and BIR-1 localize to subchromosomal foci on meiotic chromosomes. Antibody staining is presented in green and DAPI staining in red. (A-L) Representative slice images of AIR-2 (A, D, G, and J), DAPI (B, E, H, and K), or merged (C, F, I, and L) staining in oocyte-derived nuclei at different meiotic stages. (M-P) Only merged images are shown for BIR-1 at corresponding stages. Arrows in A-F and M-N point to the staining between homologues in meiosis I, and in J-L and P, they point to the staining between sister chromatids in meiosis II. Bar, 2 um.
(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).
Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.
(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.
(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.