Picture from Ofulue EN et al. (1992) DNA Cell Biol "Isolation and characterization of eft-1, an elongation factor 2-like gene on ...."

FIG. 6. Northern blot analysis. A. 5 ug poly(A)+RNA (lane 1) and 20 ug total cellular RNA (lane 3) from embryos. B. 25 ug total RNA (rows I) from all stages of development were hybridized with nick-translated 32P-labeled B3255 (panels A and BI) or with labeled pCeA7 (C. elegans actin gene 1, panel BII). E, embryo; L1-L4, larvae; D, dauer larva; A, young adult; M, size markers. Sizes of marker bands are given in kilobases.

Picture from Kramer JM et al. (1985) J Biol Chem "Expression of the Caenorhabditis elegans collagen genes col-1 and col-2 is ...."

FIG.1. Detection of collagen mRNAs synthesized during the development of C. elegant. Either 10 ng of total RNA (lanes 1-4) or 3 ng of poly(A) RNA (lanes 5-12) were electrophoresed through 1.2% agarose, 6 M formaldehyde gels and transferred to nitrocellulose filters. The stage-specific RNAs analyzed were: embryo RNA (lanes 1, 5, and 9), L2-dauer RNA (lanes 2, 6, and 10), dauer-L4RNA (lanes 3, 7, and I I ) , and L4-adult RNA (lanes 4,8, and 12). The filters were probed with nick translated plasmids. To detect the total collagen transcripts the probe was pCol-2a (lanes 1-4) used with low stringency conditions. To detect col-1 specific and col-2 specific transcripts the probes were pCol-lb (lanes5 and 6) and pCol-2b (lanes 9-12), respectively, used with high stringency conditions. The hybridized filters were exposed to XAR-5 film with intensifying screens for 22 h (lanes 1-4) or 72 h (lanes 5-12).