Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Northern blot analysis using StarFire probes detect temporal expression of mature miRNAs. E indicates embryo; L, larvae; *L1, starved L1; A, adult. Probe against the U6 snRNA was used as control.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Northern blot analysis using StarFire probes detect temporal expression of mature miRNAs. E indicates embryo; L, larvae; *L1, starved L1; A, adult. Probe against the U6 snRNA was used as control.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Northern blot analysis using StarFire probes detect temporal expression of mature miRNAs. E indicates embryo; L, larvae; *L1, starved L1; A, adult. Probe against the U6 snRNA was used as control.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Northern blot analysis using StarFire probes detect temporal expression of mature miRNAs. E indicates embryo; L, larvae; A, adult. Probe against the U6 snRNA was used as control.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Temporal miRNA promoter activity. Representative images of miRNA promoters that drive GFP expression in the embryo. (Left) DIC images; (right) GFP fluorescence.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Spatial miRNA promoter activity. (Top) DIC images; (bottom) GFP fluorescence.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Northern blot analysis using StarFire probes detect temporal expression of mature miRNAs. E indicates embryo; L, larvae; *L1, starved L1; A, adult. Probe against the U6 snRNA was used as control.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Northern blot analysis using StarFire probes detect temporal expression of mature miRNAs. E indicates embryo; L, larvae; *L1, starved L1; A, adult. Probe against the U6 snRNA was used as control.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Temporal miRNA promoter activity. Representative images of miRNA promoters that drive GFP expression in the embryo. (Left) DIC images; (right) GFP fluorescence.

Picture from Martinez NJ et al. (2008) Genome Res "Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA ...."

Figure 3. Temporal miRNA promoter activity. (A) Representative images of miRNA promoters that drive GFP expression in the embryo. (Left) DIC images; (right) GFP fluorescence. Additional images can be found in the EDGEdb database (Barrasa et al. 2007). (B) miRNAs tend to be expressed later in development compared to TFs and 'all genes.' The asterisk indicates a significant difference (P < 0.05). (C) Percentage of miRNA and TF promoters that drive expression in the embryonic stage: embryo only, early, mid, and late embryonic stages.