Figure 9. Reaction of wild-type, adult C. elegans body wall musculature with monoclonal antibody MAC 155 to Lethocerus kettin (red), in combination with monoclonal antibody DM5-6 to C. elegans myosin heavy chain A (green) in (a) and with monoclonal antibody MH24 to C. elegans vinculin (green) in (b). On the right hand side of (a), myosin in the A-bands (green) alternates with punctate labelling of kettin (red) in dense bodies. In (b), the simultaneous labelling of kettin and vinculin results in the yellow appearance of dense bodies (rows of spots). A single quadrant of multi-sarcomeric, spindle-shaped muscle cells is illustrated in (a), and two quadrants are shown in (b). Scale bar represents 20 um.
Figure 8. Reaction of wild-type, adult C. elegans with monoclonal antibody to Lethocerus kettin (MAC 155). (a) Labelling of the pharyngeal musculature. (b) Ventro- lateral view of midsection of hermaphrodite, showing labelling of the vulval muscles and the two ventral quadrants of body wall muscle. The speckled labelling towards the top of the panel arises from labelling of the uterus. (c) Posterior of hermaphrodite, revealing labelling of ventral body wall muscle quadrants and anal depressor muscle. (d) Posterior of male, showing labelling, of anal depressor muscle and proximal gonad (e.g. vas deferens) of adult male. bwm, body wall muscle; pg, proximal gonad; adm, anal depressor muscle; r, ruff of male tail fan; u, uterus; v, vulva. Scale bar represents 20 um for (a) and (d) and 40 um for (b) and (c).
(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).
Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.
(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.
(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.
CEH-20 is expressed in P11.aaap. DIC (A) and epifluorescence (B) images. (A,B) The posterior ventral nerve cord of a late L2 stage animal carrying a rescuing Pceh-20ceh-20:cfp fusion gene.