Picture from Miller DM et al. (1983) Cell "Differential localization of two myosins within nematode thick ...."

Figure 10. Antibody S-8 Reacts with the Central Region of the Thick Filament Only(A) Electron micrograph of negatively stained thick filament. (B) Low, (C) medium, and (D) high magnification images of a thick filament reacted with antibody 5-8 (1 m/ml) plus goat antimouse IgG (20 ug/ml). Pointers denote edges of antibody reacted zone. Bars in (A, B) are 1 um and bars in (C, D) are 0.2 um.

Picture from Gonzales-Moreno C et al. (2023) MicroPubl Biol "Low-dose N-acetyl cysteine prevents paraquat-induced mortality in ...."

Figure 1. Survival expressed as a percent of lethality:A. Effect of one-hour NAC exposure on L4- worms' survival. *p<0.05; ***p<0.001 respect to the control group. B. Lethality measured after one-hour PQ exposure preceded by one-hour 0.5 mM NAC treatment (immediate toxicity assay). **p<0.01; ***p<0.001 with respect to the control group, and ##p<0.01 and ###p<0.001 related to the respective PQ-exposure concentration. C. Lethality measured 24 hours after one-hour PQ exposure preceded by one-hour 0.5 mM NAC treatment (delayed toxicity assay). ***p<0.001 with respect to the control group, and ###p<0.001 related to the respective PQ-exposure concentration. The dots indicate the number of assays averaged from tests performed in triplicate. The bars show the percentage of dead animals + the standard error.

Picture from Stobierska J et al. (2025) MicroPubl Biol "Low-fecundity dhc-1; mel-28 C. elegans mutants do not have gonad mitosis ...."

Figure 1. dhc-1; mel-28 double mutant gonads resemble the wild type with regard to length and mitotic activity: (A) Gonads from live wild-type animals, mel-28 mutants, dhc-1 mutants, and dhc-1; mel-28 double mutants. Red marks the chromatin and green marks the cell membranes. Z-stacks were captured and then collapsed using the Keyence microscope Analyzer software. The dotted lines indicate how we measured the length of the gonads. Scale bar = 50 μM(B) Fixed distal gonads from wild-type animals, mel-28 mutants, dhc-1 mutants, and dhc-1; mel-28 double mutants. The blue represents DAPI staining, the red indicates nuclear pores, and the green indicates mitotic nuclei. Z stacks were captured and collapsed, and images were processed using haze-reduction software from the Keyence Analyzer package. Scale bar = 50 μM.(C) Gonad arm length measurements. At least 20 gonads of each strain were measured. Pairwise t tests between strains yielded p values > 0.05, except for the comparison between mel-28 mutant gonads and dhc-1 mutant gonads, which returned a p value of 0.014.(D) PH3-positive nuclei counts. At least 60 gonads of each genotype were analyzed for mitotic activity. Pairwise t tests between the strains yielded p values > 0.05, except for the comparison between dhc-1 mutants and the wild-type which showed a p value of 0.048.

Picture from Barriere A et al. (2012) PLoS Genet "Coevolution within and between regulatory loci can preserve promoter function ...."

Figure 1. The expression pattern of unc-47 is conserved despite divergent regulation. (A) Both C. elegans and C. briggsae promoters fused upstream of GFP in their endogenous trans-regulatory environments drive expression in all 26 GABAergic neurons (green). However, the C. briggsae promoter placed in the C. elegans trans environment additionally drives expression in SDQR and SDQL (blue). (B) Those cells were identified as SDQR/L based on their position and their characteristic projections. (C) For each combination of promoter and trans-regulatory environment, expression in SDQR and SDQL is presented. C. elegans is represented by straight lines, C. briggsae by wavy lines. Frequency of expression is represented by the width, and intensity of expression relative to D-type neurons by the height of black boxes. Number of individuals expressing and total number of individuals scored is indicated underneath. Measurements for independent strains are given in Figure S1. Differences in frequency of expression in SDQR are significant for all comparisons: C. elegans and C. briggsae promoters in C. elegans trans environment (p = 8.1610212), C. elegans and C. briggsae promoters in C. briggsae trans environment (p = 1.561028), C. elegans promoter in C. elegans and C. briggsae trans environments (p = 4.8610211), C. briggsae promoter in C. elegans and C. briggsae trans environments (p = 2.4610214). (D) Interpretation of the results presented in panel C. Both C. elegans and C. briggsae promoters in their endogenous trans environments drive low levels of expression in SDQR and SDQL, while disruption of the endogenous interactions either drives high levels of expression (C. briggsae promoter in C. elegans) or abolishes expression (C. elegans promoter in C. briggsae).

Picture from Beaster-Jones L et al. (2004) J Mol Biol "DNA binding and in vivo function of C.elegans PEB-1 require a conserved FLYWCH ...."

Figure 2. Cb-PEB-1 and Ce-PEB-1 are expressed in similar pattern. Fluorescence micrographs of 1.5-fold stage C. briggsae (A) and C. elegans embryos (B) stained with C. elegans PEB-1 antibodies (anterior is left). Staining is most abundant in pharyngeal nuclei in both C. briggsae and C. elegans (brackets). Fainter staining is visible in hypodermal cells and the hindgut in C. elegans and C. briggsae, although all staining was generally weaker in C. briggsae.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(C, C') Pgrl-2::GFP is expressed in the excretory duct and pore cells.

Picture from Barkoulas M et al. (2016) PLoS Genet "Evolution of New cis-Regulatory Motifs Required for Cell-Specific Gene ...."

(B-E) smFISH using a lin-3 probe in C. elegans N2 (data from [2]) (B), C. briggsae AF16 (C), C. angaria RGD1 (D) and O. tipulae CEW1 (E). Red arrow marks the position of the anchor cell.

Picture from Hobert O et al. (1999) J Cell Biol "A conserved LIM protein that affects muscular adherens junction integrity and ...."

(A-C): UNC-97::GFP localization in live worms at embryonic stage 300 min (A), 400 min (comma stage) (B), and 500 min (threefold pretzel stage) (C). (A'-C') Corresponding DIC micrographs. White arrows in C, a muscle nucleus and dense bodies, respectively. Note UNC-97::GFP localization to the periphery of nuclei in A-C.

Picture from Vazquez-Manrique RP et al. (2007) Genomics "The frataxin-encoding operon of Caenorhabditis elegans shows complex ...."

(a-c) Expression of F59G1.1::gfp in pharynx and gut (a), anal cells (b), and spermatheca (sph) (c).

Picture from Cui Y et al. (2009) J Biol Chem "Cadmium induces retinoic acid signaling by regulating retinoic acid metabolic ...."

bcmo-1::GFP expression in post-embryonic C. elegans. Images of untreated C. elegans (A, C, and E). L1 (A) and L4 (C) larvae are at 400x and 200x magnification, respectively. Adult C. elegans (E) is a composite of images acquired at 200x magnification. Arrowhead, indicates the location of a bcmo-1::GFP expressing embryo in a gravid adult nematode.