Figure 3. Expression of
mir-48::gfp and
mir-241::gfp Fusions. (A)
mir-48 and
mir-241 gfp fusions are diagrammed. Gray arrows indicate the GFP coding sequence. 'AT' indicates that the inverted repeat was replaced with an AT-rich sequence. The asterisk indicates the
ve33 lesion.(B-R) Representative gfp expression patterns. Where especially relevant, exposure times are indicated in the panels (exp). (B)
mir-48::gfp expression is not detected in L3 molt animals, but is readily detected at the (C) L4 molt. (D and E) The
ve33 lesion in
mir-48(
ve33)::gfp results in enhanced gfp expression that is detectable one stage earlier. (F-I) gfp is expression in descendents of P5.p and P7.p in the vulva. Corresponding Nomarski DIC images are shown in (K)-(N).
mir-48::gfp is not detected at the L3 molt ([F]; exp = 2.0 s), but is present at the L4 molt ([G]; exp = 1.0 s).
mir-48(
ve33)::gfp is detected in the L3 stage ([H]; exp = 0.5 s) and more strongly at the L4 molt ([I]; exp = 0.06 s). (J and O) L2-stage RID expression in
mir-48::gfp and
mir-48(
ve33)::gfp, respectively. (P-R)
mir-241::gfp is expressed in a variety of cell types as indicated for the (P) L3 and (R) L2 stages. (Q) is the Nomarski DIC image corresponding to (P). The bar in (B) is 10 um and applies to all panels except (J) and (O); the bar in (J) is 20 um. Stage-nonspecific expression was observed in unidentified neurons in the head, tail, and ventral nerve cord for both
mir-48 and
mir-241 constructs.