Figure 1. AQR and PQR migration defects in
dpy-17,
sqt-3,
dpy-14, and
bli-4 mutants: A-D) Fluorescent and DIC merged images of animals with Pgcy-32::cfp expression (cyan). The URX, AQR, and PQR neurons are indicated. The scale bar in A represents 5 μm. Genotype names of
dpy-17(
syb7589) and
sqt-3(
syb7590) were shortened for space and do not include the
dpy-17(
syb3685) and
sqt-3(
syb3691) gfp insertions also in the strains. Images were acquired in the cfp fluorescence channel, so GFP from the gene tags is not visible in the figures. M+ indicates that the animal had wild-type maternal gene activity (i.e. was a homozygous mutant progeny of a heterozygous mother). A) In wild-type, AQR is in the head deirid ganglion (position 1), and PQR is in the phasmid ganglion posterior to the anus (position 5). B) Both AQR and PQR migrated posteriorly to the phasmid ganglion in a
dpy-17(
syb7589) mutant. C) Both AQR and PQR migrated anteriorly to the deirid ganglion in a
sqt-3(
syb7590) mutant. D) Both AQR and PQR migrated posteriorly in a
bli-4(
cs302) mutant. E) A table showing the positions of AQR and PQR in different genotypes. For each genotype, 100 animals were scored.
dpy-17(
e164) and
sqt-3(
e2924) data taken from (Lang and Lundquist 2021). The asterisk (*) represents p ≤ 0.001 compared to the null allele at that position (Fisher's exact test). The diagram above the table represents the five positions along the anterior-posterior axis of the animal. Q neuroblasts are born in position 4. CFCS indicates a consensus furin cleavage site mutant. CRD represents a cysteine rich domain containing isoform mutant. CRD TM represents a mutant in the isoforms containing the cysteine-rich domain and transmembrane domain. BLI indicates a mutation in the viable Bli phenotype isoforms.