Picture from Ambros V et al. (2003) Curr Biol "MicroRNAs and other tiny endogenous RNAs in C. elegans."

Northern Blot Hybridization. Panel(B) shows the 22 nt (mature miRNA) size range. In panel B the lanes contain total RNA samples from (left to right): lane 1, embryos; lane 2, L1 larvae; lane 3, L2 larvae; lane 4, L3 larvae; lane 5, L4 larvae; and lane 6, adults. In panel D the lanes contain total RNA samples from (left to right): lane 1, embryos; lane 2, L1 larvae held in developmental arrest for 24 hours by starvation; lane 3, L1 larvae 12 hours after feeding; lane 4, L1 larvae 18 hours after feeding.

Picture from Ambros V et al. (2003) Curr Biol "MicroRNAs and other tiny endogenous RNAs in C. elegans."

Northern Blot Hybridization. Panel (A) shows the 22 nt (mature miRNA) size range. The lanes contain total RNA samples from (left to right): lane 1, embryos; lane 2, L1 larvae; lane 3, L2 larvae; lane 4, L3 larvae; lane 5, L4 larvae; and lane 6, adults.

Picture from Ambros V et al. (2003) Curr Biol "MicroRNAs and other tiny endogenous RNAs in C. elegans."

Northern Blot Hybridization. Panel C and G show the 22 nt (mature miRNA) size range and the 65 nt (miRNA precursor) range. In panel C, the lanes contain total RNA samples from (left to right): lane 1, embryos; lane 2, L1 larvae; lane 3, L2 larvae; lane 4, L3 larvae; lane 5, L4 larvae; and lane 6, adults. In panel G, the lanes contain total RNA samples from (left to right): lane 1, embryos; lane 2, L1 larvae held in developmental arrest for 24 hours by starvation; lane 3, L1 larvae 12 hours after feeding; lane 4, L1 larvae 18 hours after feeding.

Picture from Ono K et al. (2003) J Cell Sci "Specific requirement for two ADF/cofilin isoforms in distinct actin-dependent ...."

Figure 1. Expression of mRNAs for UNC-60A and UNC-60B during C. elegans development. 25 ug of total RNA from the indicated stages was loaded on each lane and probed with radiolabeled cDNAs for unc-60A, unc-60B or 18S rRNA. Samples are embryos (lane 1), L1 larvae (lane 2), L2 larvae (lane 3), L3 larvae (lane 4), L4 larvae (lane 5), young adults (lane 6) and gravid adults (lane 7).

Picture from Yang J et al. (1999) J Biol Chem "Proteolytic processing of Caenorhabditis elegans SQT-1 cuticle collagen is ...."

Figure 5. Western blot analyses of wild-type cuticle extracts from animals at different developmental stages. Cuticle extracts examined were: lane 1, L1 larvae; lane 2, L2 larvae; lane 3, L3 larvae; lane 4, L4 larvae; lane 5, adults; lane 6, dauer larvae;lane 7, animals in the L4-adult molt. The blot was reacted with affinity purified anti-s1c antisera. Molecular mass standards are indicated in kilodaltons to the left.

Picture from Ambros V et al. (2003) Curr Biol "MicroRNAs and other tiny endogenous RNAs in C. elegans."

Figure 3. Examples of Developmental Patterns of Expression of Worm MicroRNAs Detected by Northern Blot Hybridization. Panels (A), (B), and (D-F) show the 22 nt (mature miRNA) size range, and panels (C) and (G) also include the 65 nt (miRNA precursor) range. In panels (A)-(C), the lanes contain total RNA samples from (left to right): lane 1, embryos; lane 2, L1 larvae; lane 3, L2 larvae; lane 4, L3 larvae; lane 5, L4 larvae; and lane 6, adults. In panels (D)-(G), the lanes contain total RNA samples from (left to right): lane 1, embryos; lane 2, L1 larvae held in developmental arrest for 24 hours by starvation; lane 3, L1 larvae 12 hours after feeding; lane 4, L1 larvae 18 hours after feeding.

Picture from Starich TA et al. (1993) Genetics "Molecular and genetic analysis of unc-7, a Caenorhabditis elegans gene required ...."

FIGURE 5. Transcript levels of unc-7 vary over the course of development. RNA was isolated from eggs (lane 1). the four larval stages LI (lane 2). L2 (lane 3).L3 (lane 4). and L4 (lane 5),and young adults(lane6). A northern blot containing total RNA, 30pg per lane, was hybridized to unc-7 coding sequences (Ao) r an rRNA probe (B, showing 18s signal). Poly(A)-enriched RNA, 10 ug per lane (C), was hybridized to the same unc-7 probe as in A.

Picture from Wu Y et al. (2001) J Biol Chem "Expression and secretion of a larval-specific chitinase (family 18 glycosyl ...."

Figure 7. Expression pattern of Ov-CHI-1 homologue in C. elegans. A, immunoblot of C. elegans life cycle stages probed with rabbit antiserum to recombinant C. elegans chitinase. Samples from left to right are as follows:lane 1, 3-day-old hermaphrodite; lane 2, L4 larvae; lane 3, L2-L3 larvae; lane 4, L1 larvae;lane 5, dauer larvae; lane 6, laid eggs.B, a panel of the corresponding samples probed with preimmune rabbit serum.

Picture from Detwiler MR et al. (2001) Dev Cell "Two zinc finger proteins, OMA-1 and OMA-2, are redundantly required for oocyte ...."

(A) Extracts of bacteria expressing no fusion protein (lane 1), GST-OMA-1 (lane 2), or GST-OMA-2 (lane 3), and whole-worm extracts from wild-type N2 (lane 4), oma-1(te33) (lane 5), and oma-2(te51) (lane 6) were probed with antibodies to OMA-1, OMA-2, or actin.(B) Worm lysates from wild-type N2, glp-4(bn2), fem-3(q20sd), or fem-3(e2006) grown at either 15C or 25C probed with antibodies to OMA-1, OMA-2, or actin.

Picture from Ding L et al. (2000) J Biol Chem "HSP25, a small heat shock protein associated with dense bodies and M-lines of body ...."

Figure 5. Expression of HSP25. A, effect of heat shock on HSP25 synthesis. C. elegans was cultured either at 20 C (lane 1) or heat shocked at 33 C (lane 2). Extracts were analyzed by SDS-PAGE and detected by Western blotting (upper panel, antibody to HSP25; lower panel, antibody to actin). B, extracts were prepared from C. elegans at each developmental stage and analyzed by SDS-PAGE and Western blotting. Lane 1, embryo; lanes 2-5, L1 to L4 larval stages; lane 6, adult; lane 7, recombinant HSP25. Extract loadings were normalized to actin levels (B, lower panel).