Picture from Hsu TY et al. (2010) Curr Biol "Engulfment of apoptotic cells in C. elegans is mediated by integrin alpha/SRC ...."

Figure S2. INA-1 Is Widely Expressed during Embryogenesis. INA-1::GFP (a) and DIC (b) images of a ced-5(n1812) embryo expressing Pina-1 ina-1::gfp at the comma stage. An apoptotic cell is indicated by an arrow and shown in the inset. The empty arrows show the developing gut with a much weaker INA-1::GFP signal.

Picture from Baum PD et al. (1997) Neuron "Neuronal migrations and axon fasciculation are disrupted in ina-1 integrin ...."

INA-1 Expression Pattern. (A-C) Anti-INA-1 immunofluorescence.(A) Lateral view of a 1.5-fold embryo, approximately midway through embryogenesis. The developing head is to the left, and the tail is to the right. Although all cells express a low level of INA-1, there is increased staining in the region of the developing pharynx (arrowheads).(B) Lateral view of a late stage embryo. The pharynx still stains brightly (arrowheads). The intestinal staining (arrows) is not due to INA-1 protein because it persists in ina-1(gm86) molecular null mutants ([C], arrows).

Picture from Meighan CM et al. (2015) Gene "Transcription factor hlh-2/E/Daughterless drives expression of integrin ...."

Fig. 1. 400 bp of ina-1 promoter is sufficient for GFP expression in the DTC. (A) DTC migration in the anterior of the nematode body. DTC migration starts at the vulva and moves along the ventral surface of the nematode body cavity. The DTC then moves to the dorsal surface where it turns again to migrate back to the mid-region of the nematode body. JE1111, ina-1p::RFP, has normal DTC migration along the dorsal surface of the nematode (A) and expression of RFP (B). ina-1p1000::GFP (C), ina-1p400::GFP (E), and ina-1p336::GFP (G) fusions of the ina-1 promoter to GFP all have DTC migration on the dorsal surface. Ina-1p1000::GFP (D) and ina-1p400::GFP (F) fusions have GFP expression. (H) ina-1p336::GFP has no GFP expression. A schematicof each transgene appears to the left of the related images; lines represent the ina-1 promoter, boxes represent the RFP or GFP gene as indicated. Dashed lines outline the gonad arm and arrows point to the DTCs in each image. Anterior is to the left. The ventral gonad arm was not outlined in F and H for clarity. Scale bar, 25 um.

Picture from Baum PD et al. (1997) Neuron "Neuronal migrations and axon fasciculation are disrupted in ina-1 integrin ...."

Figure 6. INA-1 Expression Pattern. (A-C) Anti-INA-1 immunofluorescence.(A) Lateral view of a 1.5-fold embryo, approximately midway through embryogenesis. The developing head is to the left, and the tail is to the right. Although all cells express a low level of INA-1, there is increased staining in the region of the developing pharynx (arrowheads).(B) Lateral view of a late stage embryo. The pharynx still stains brightly (arrowheads). The intestinal staining (arrows) is not due to INA-1 protein because it persists in ina-1(gm86) molecular null mutants ([C], arrows).(D and E) L1 larvae bearing the ina-1-GFP reporter construct gmIs5. Anterior is to the left, and ventral is down.(D) INA-1-GFP is expressed in the migrating Q cell lineage, beginning with the initial migrations of the Q cells. Here, the QR and QL daughters are shown. The photomicrograph shows the right side of the animal, with the left side of the same animal in the inset.(E) Neuronal expression of INA-1-GFP is seen in the head and ventral nerve cord (arrowheads). The scale bar is 10 μm.

Picture from Hsu TY et al. (2010) Curr Biol "Engulfment of apoptotic cells in C. elegans is mediated by integrin alpha/SRC ...."

(Ba-Bd) INA-1::GFP and SRC-1::mCherry are colocalized to the pseudopods extending around an apoptotic cell. INA-1::GFP (Ba), SRC-1::mCherry (Bb), merged INA-1::GFP and SRC-1::mCherry (Bc), and DIC (Bd) images of the same embryo coexpressing Pced-1ina-1::gfp and Pced-1src-1::mcherry are shown. Apoptotic cells are indicated by arrows and are magnified in the insets. Scale bar in (Ba) represents 5 um.

Picture from Baum PD et al. (1997) Neuron "Neuronal migrations and axon fasciculation are disrupted in ina-1 integrin ...."

INA-1 Expression Pattern. (D and E) L1 larvae bearing the ina-1-GFP reporter construct gmIs5. Anterior is to the left, and ventral is down.(D) INA-1-GFP is expressed in the migrating Q cell lineage, beginning with the initial migrations of the Q cells. Here, the QR and QL daughters are shown. The photomicrograph shows the right side of the animal, with the left side of the same animal in the inset.(E) Neuronal expression of INA-1-GFP is seen in the head and ventral nerve cord (arrowheads). The scale bar is 10 μm.

Picture from Hsu TY et al. (2010) Curr Biol "Engulfment of apoptotic cells in C. elegans is mediated by integrin alpha/SRC ...."

Figure 2. INA-1 Recognizes Apoptotic Cells and Is Required for Their Internalization by Engulfing Cells. (Aa-Aj) INA-1::GFP is enriched in the pseudopods extending around apoptotic cells. Time-lapse images of INA-1::GFP (Aa-Ae) and differential interference contrast (DIC; Af-Aj) during cell corpse internalization in an embryo expressing Pced-1ina-1::gfp are shown. 0 min indicates the time point at which INA-1::GFP was first seen to accumulate at the contact site with C3. Arrowheads indicate the apoptotic cell (C3); arrows indicate the engulfing cell (ABplaapppp). Scale bar in (Aa) represents 5 μm.(Ba-Bc) ina-1 mutations reduce the efficiency of cell corpse internalization in engulfing cells.(Ba and Bb) MYRI::GFP and DIC images of wild-type (Ba) and ina-1(gm144) (Bb) embryos expressing Pced-1myri::gfp. Arrows indicate apoptotic cells. Scale bar in (Ba) represents 5 μm.(Bc) Percentage of apoptotic cells with a MYRI::GFP circle in the indicated genotype. Data (mean ± SD) are the average for two independent transgenic lines. Numbers in parentheses indicate the number of cell corpses scored.(Ca-Cc) INA-1(N) binds to the surface of apoptotic cells in a scrm-1-dependent manner.(Ca and Cb) INA-1(N)::GFP and DIC images of ced-1(e1735); ced-5(n1812) (Ca) and scrm-1(RNAi)ced-1(e1735); ced-5(n1812) (Cb) embryos expressing Phspina-1(N)::gfp. Arrows indicate apoptotic cells. Scale bar in (Ca) represents 5 μm.(Cc) Percentage of INA-1(N)::GFP+ cell corpses in the indicated genotype. Cell corpses fully enclosed by the INA-1(N)::GFP circle were scored as INA-1(N)::GFP+. Each sample point represents the data from 12 embryos. Data are shown as mean ± SD.

Picture from Hsu TY et al. (2010) Curr Biol "Engulfment of apoptotic cells in C. elegans is mediated by integrin alpha/SRC ...."

Figure 3. SRC-1 Physically Interacts with INA-1(i) and CED-2 In Vitro and Is Colocalized with INA-1 in Phagocytes to the Pseudopods Extending around Apoptotic Cells. (A) SRC-1 interacts with INA-1(i) and CED-2 in the yeast two-hybrid assay. Pairs of constructs expressing the indicated fusion proteins were transformed into the yeast strain MaV203. The resulting transformants were streaked on SC-Trp-Leu-His or SC-Trp-Leu plates containing 25 mM 3AT. Growth on the SC-Trp-Leu-His + 25 mM 3AT plate indicates interaction of fusion proteins.(Ba-Bd) INA-1::GFP and SRC-1::mCherry are colocalized to the pseudopods extending around an apoptotic cell. INA-1::GFP (Ba), SRC-1::mCherry (Bb), merged INA-1::GFP and SRC-1::mCherry (Bc), and DIC (Bd) images of the same embryo coexpressing Pced-1ina-1::gfp and Pced-1src-1::mcherry are shown. Apoptotic cells are indicated by arrows and are magnified in the insets. Scale bar in (Ba) represents 5 um.

Picture from Hsu TY et al. (2010) Curr Biol "Engulfment of apoptotic cells in C. elegans is mediated by integrin alpha/SRC ...."

(Aa-Aj) INA-1::GFP is enriched in the pseudopods extending around apoptotic cells. Time-lapse images of INA-1::GFP (Aa-Ae) and differential interference contrast (DIC; Af-Aj) during cell corpse internalization in an embryo expressing Pced-1ina-1::gfp are shown. 0 min indicates the time point at which INA-1::GFP was first seen to accumulate at the contact site with C3. Arrowheads indicate the apoptotic cell (C3); arrows indicate the engulfing cell (ABplaapppp). Scale bar in (Aa) represents 5 um.

Picture from Meighan CM et al. (2007) Genes Dev "Control of C. elegans hermaphrodite gonad size and shape by vab-3/Pax6-mediated ...."

Figure 4. Ina-1 expression is necessary for normal and perpetual DTC migration. Images of strain NG2517, an INA-1::GFP translational fusion, show diminishing GFP fluorescence between L4 (A) and adult DTCs (B). (C) INA-1::GFP is maintained throughout egg-laying in the adult vab-3 mutants. (D,E) Gonads were dissected from age-matched NG2517 wild-type or vab-3 mutants expressing INA-1::GFP. Fluorescence images were captured at identical settings. Arrows point to DTCs (A-E), and arrowheads point to vulva (A,B). RNAi against ina-1 on N2 (F) or vab-3(e1796) (G) nematodes stops DTC migration. Simultaneous RNAi against ced-10 and mig-2 (H) on a vab-3 mutant prevents perpetual migration. Dashed lines trace gonad arms, and arrowheads mark the DTCs. Bars: all panels except D,E, 25 μm; D,E, 12.5 μm.