Picture from Gill JP et al. (2024) MicroPubl Biol "Human SVIP is sufficient to stimulate tubular lysosomes and extend ...."

Figure 1. Human SVIP induces TLs constitutively, increases autophagic activity, and improves healthspan when expressed in the C. elegans gut: a. Endogenously tagged SPIN-1::mCherry in fed adult day 1 WT, dSVIP gut OE, and hSVIP gut OE worms (scale bar, 5μm). b. Quantification of lysosomal junctions/object in fed adult day 1 WT (n=15 worms), dSVIP gut OE (n=15 worms), and hSVIP gut OE (n=14 worms) worms (p-values indicated on graph). c. Gut-expressed SQST-1::mCherry::GFP in fed adult day 2 WT, dSVIP gut OE, and hSVIP gut OE worms (scale bar, 100μm). d. GFP/mCherry ratio in fed adult day 2 WT (n=11 worms), dSVIP gut OE (n=13 worms), and hSVIP gut OE (n=15 worms) worms (p-values indicated on graph). e. Gut-expressed Q64::YFP in fed adult day 1 WT, dSVIP gut OE, and hSVIP gut OE (scale bar, 100μm). f. Number of Q64::YFP aggregates in fed adult WT, dSVIP gut OE, and hSVIP gut OE worms on day 1 of adulthood (n=10 for each genotype; p-values indicated on graph). g. Thrashing rate of fed adult WT, dSVIP gut OE, and hSVIP gut OE worms (n=10 worms for each genotype; p-values on graph are comparisons to WT, **p<0.01, ***p<0.001, ****p<0.0001). h. Lifespan of fed adult WT, dSVIP gut OE, and hSVIP gut OE worms. i. Descriptive statistics for the lifespan comparison in Fig. 1h. j. Log-rank test results for lifespan comparisons in Fig. 1h.