4 Pictures found (0.071 seconds)

Picture from Martin E et al. (2014) PLoS One "pix-1 controls early elongation in parallel with mel-11 and let-502 in ...."

Picture from Lee KK et al. (2002) Mol Biol Cell "Lamin-dependent localization of UNC-84, a protein required for nuclear ...."

Figure 6. UNC-84 localization during mitosis. Wild-type embryos (100-300 cells) were stained with rat anti-UNC-84 antibodies (right panels) and Hoechst 33258 to stain DNA (left panels), viewed by fluorescence microscopy and photographed at different stages of the cell cycle. A representative nucleus from each stage is shown: IP, interphase; EP, early prophase; LP, late prophase; PM, prometaphase; MT, metaphase; EA, early anaphase; LA, late anaphase and TL, telophase. Nuclear staining was detected in all stages of mitosis except late anaphase, consistent with other nuclear membrane markers (Lee et al., 2000).

Picture from Martin E et al. (2014) PLoS One "pix-1 controls early elongation in parallel with mel-11 and let-502 in ...."

Figure 5. PIX-1::GFP is differentially expressed in hypodermal cells during elongation. A) Confocal lateral projections of pix-1(gk416);sajEx1[pix-1p::pix-1::GFP]; mcIs40 [lin-26p::ABDvab-10::mcherry + myo-2p::gfp] embryos. Dorsal-anterior (DA, upper panel), dorsal-posterior (DP, upper panel), ventral-anterior (VA, upper panel), ventral-posterior (VP, upper panel), lateral-anterior (LA, lower panel) and lateral-posterior (LP, lower panel) hypodermis are surrounded by dashed line and have been identified using lin-26p::vab-10(ABD)::MCHERRY hypodermal markers. B) Distributions ofthe dorsal-posterior/dorsal-anterior (DP/DA), lateral-posterior/lateral-anterior (LP/LA), ventral-posterior/ventral-anterior (VP/VA), dorsal-posterior/ventral (DP/V); dorsal-anterior/ventral (DA/V), dorsal-anterior/lateral (DA/L) rates of fluorescence intensity measured in pix-1(gk416); sajEx1[pix-1p::pix1::GFP; rol-6]; mcIs40 [lin-26p::ABDvab-10::mcherry + myo-2p::gfp] embryos between comma and 1.75-fold stages (n = 26 embryos). Similar results were also obtained in pix-1(gk416); sajIs1[pix-1p::pix-1::GFP; unc-119R]; mcIs40 [lin-26p::ABDvab-10::mcherry + myo-2p::gfp]. The box-plots represent the min, max, 25th, 50th (median) and 75th percentiles of the populations. ** T-test comparing ratios to 1 p,0.01. C) Schematic representation of pix-1::GFP and ABDVAB-10 (control) constructs used to measure the DP/DA intensity ratio reported in panel D. DP/DA of animals carrying mcIs40 (lin-26p::ABD::mCh expressing), mcIs50 (lin-26p::ABD::GFP expressing), sajIs2 (lin-26p::pix-1::GFP expressing) or sajEx1 (pix-1p::pix-1::GFP expressing). ratios** T-test comparing DP/DA ratios measured on pix-1::GFP expressing embryos to ratio measured in ABDVAB-10 expressing transgenics, p,0.01. The box-plotsrepresent the min, max, 25th, 50th (median) and 75th percentiles of populations.

Picture from Land M et al. (1994) J Biol Chem "Structure and expression of a novel, neuronal protein kinase C (PKC1B) from ...."

Fig.10. Expression of PKC1B in situ. C elegans were processed for immunofluorescence as described under 'Experimental Procedures'. Specimens were analyzed by confocal microscopy (see 'Experimental Procedures'). Panel A (right) shows the head region. Cell bodies and processes of neurons of the nerve ring (NR), anterior (AG), dorsal, lateral, ventral (DLV)and retrovesicular (R) ganglia, amphid (A) and labial (LA) sensory bundles exhibit intense fluorescence due to PKC1B.IgG complexes. Neuronal processes in ventral nerve cord (V), touch cell interneurons (B,S), and neurons of the posterior lateral ( P ) ganglion are evident in panel A (left) and panel B. Cell bodies and processes of the tail ganglia, the phasmid neurons, and dendrites of touch receptor neurons are also enriched in PKC1B(D, E, F, panelA (left)). B and S are interneurons designated BDUL/R and SDQL/R, respectively. D indicates the dorsorectal ganglion; E, the lumbar gan- glion; and F, the preanal ganglion.