Picture from Dong J et al. (2008) J Mol Biol "Molecular characterization of two homologs of the Caenorhabditis elegans ...."

Fig. 6. Intracellular location of CDR-4 in vivo. Transgenic C. elegans that contain a cdr-4/CDR-4-eGFP expression vector were fed RITC-dextran. The location of CDR-4-eGFP (GFP) and RITC-labeled lysosomes (Rhodamine) was visualized using the appropriate filter sets. A false-color, composite image (Merge) that demonstrates the localization of CDR-4 in lysosomes is presented. A higher magnified (400) image of the identical transgenic C. elegans is presented in the inset. Locations where the eGFP and rhodamine signals are coincident can be identified by the yellow color. The lowermost panel indicates the location of CDR-4-eGFP within the pharynx of an adult C. elegans. The location of pharyngeal muscle cells pm5, pm6, and pm7 is indicated. pm5 cells are columnar cells, which extend from the anterior region of the terminal bulb to the posterior of the metacorpus.

Picture from Dong J et al. (2008) J Mol Biol "Molecular characterization of two homologs of the Caenorhabditis elegans ...."

Fig. 4. Cell-specific and developmental expression of cdr-4. Transgenic C. elegans containing pcdr-4/lacZ were stained for b-galactosidase activity. The typical expression pattern of cdr-4 in an adult (a), L1 larva (b), and embryo (c) is shown. An enlarged view of an adult C. elegans pharynx with pharyngeal muscle nuclei identified (d).

Picture from Dong J et al. (2002) J Biol Chem "Molecular characterization of a novel, cadmium-inducible gene from the ...."

Figure 6. Cell-specific expression of cdr-1. A and B, C. elegans, strain JF9 (mtIs7, cdr-1/lacZ) was exposed to 100 uM CdCl2 for 24 h and then stained for beta-galactosidase activity as previously described (19). A, reporter gene activity in the intestinal cells of a young adult and an L3 larva. B, cdr-1 promoter activity in the intestinal cells of an L1 larva. C, in situ hybridization of cadmium-treated L1 C. elegans larvae. CDR-1 mRNA was visualized by exposing wild type nematodes to a digoxigenin- dUTP-labeled CDR-1 antisense probe as described under Experimental Procedures. C shows that the CDR-1 transcript is expressed throughout the intestine of L1. ph marks the location of the pharynx in all panels. Nematodes were photographed using Nomarski optics as described previously.

Picture from Dong J et al. (2002) J Biol Chem "Molecular characterization of a novel, cadmium-inducible gene from the ...."

FIG. 8. Intracellular location of the CDR-1 protein in vivo. Transgenic C. elegans, which contain a cdr-1/CDR-1-eGFP expression vector (JF13 (mtEx10, cdr-1/CDR-1-GFP)), were fed RITC-dextran and then exposed to cadmium as described under 'Experimental Procedures.' Upper panels, JF13 (mtEx10, cdr-1/CDR-1-GFP) C. elegans L2 larva. 'L' and 'Ph' indicate the location of the intestinal lumen and the nematode pharynx, respectively. Left panel, the location of CDR-1 visualized by fluorescent microscopy. Right panel, RITC-labeled lyso- somes visualized using a rhodamine filter set. Lower panel, composite, high magnification (1000x) view of an adult JF13 (mtEx10, cdr-1/CDR- 1-GFP) C. elegans. The nematode is oriented with the gonad on the left and the intestine on the right. Fluorescence images obtained using eGFP and rhodamine filter sets were superimposed on the light microphotograph. Locations where the eGFP and rhodamine signals are coincident can be identified by the blue color. Black arrows indicate eGFP and rhodamine-containing lysosomes. White arrows indicate non-lysosomal gut granules.

Picture from Dong J et al. (2002) J Biol Chem "Molecular characterization of a novel, cadmium-inducible gene from the ...."

FIG. 6. Cell-specific expression of cdr-1. A and B, C. elegans, strain JF9 (mtIs7, cdr-1/lacZ) was exposed to 100 uM CdCl2 for 24 h and then stained for b-galactosidase activity as previously described (19). A, reporter gene activity in the intestinal cells of a young adult and an L3 larva. B, cdr-1 promoter activity in the intestinal cells of an L1 larva. C, in situ hybridization of cadmium-treated L1 C. elegans larvae. CDR-1 mRNA was visualized by exposing wild type nematodes to a digoxigenin-dUTP-labeled CDR-1 antisense probe as described under 'Experimental Procedures.' C shows that the CDR-1 transcript is expressed throughout the intestine of L1. 'ph' marks the location of the pharynx in all panels. Nematodes were photographed using Nomarski optics as described previously (19).

Picture from Dong R et al. (2018) J Cell Biol "The inositol 5-phosphatase INPP5K participates in the fine control of ER ...."

(A) Representative confocal images of a transgenic worm expressingmCherry::SP12 and CIL-1::GFP driven by the hypodermal cell specific promoter. Scale bar: 10 um. Thepattern of CIL-1::GFP fluorescence is very similar tothe pattern of the ER marker mCherry::SP12. Insetsshowing magnified view of the boxed region (scalebar: 2 um).

Picture from Dong R et al. (2018) J Cell Biol "The inositol 5-phosphatase INPP5K participates in the fine control of ER ...."

Figure 7. The SKICH domain is essential forCIL-1 ER localization. (A) Representative confocal images of a transgenic worm expressingmCherry::SP12 and CIL-1::GFP driven by the hypodermal cell specific promoter. Scale bar: 10 um. Thepattern of CIL-1::GFP fluorescence is very similar tothe pattern of the ER marker mCherry::SP12. Insetsshowing magnified view of the boxed region (scalebar: 2 um). (B) Representative confocal imagesof transgenic worms expressing full-length CIL1::GFP or a truncated from of CIL-1 lacking the SKICH domain, CIL-1(deSKICH)::GFP. While WT CIL-1has the reticular distribution in cells expected foran ER protein, the truncated protein has a diffusecytosolic localization. Scale bar: 10 um.

Picture from Inoue T et al. (2004) Proc Natl Acad Sci U S A "Type II platelet-activating factor-acetylhydrolase is essential for ...."

(I and J) paf-2::GFP expression in embryos (lateral view, dorsal is up). Nomarski micrographs (I) and corresponding paf-2::GFP expression (J). paf-2 reporter gene constructs are expressed in epidermal cells (J, arrowheads) and intestinal cells (J, arrow) at the beginning of elongation. Anterior is to the left. (Bar, 10 um.)

Picture from Solari F et al. (1999) Development "The Caenorhabditis elegans genes egl-27 and egr-1 are similar to MTA1, a member of ...."

(J,K) egl-27::gfp expression in 1.5-fold embryo (J) and L3 hermaphrodite (K). All somatic cells appear to express the reporter gene.

Picture from Zhang D et al. (2012) J Cell Biol "RAB-6.2 and the retromer regulate glutamate receptor recycling through a ...."

Figure 2. RAB-6.2 is broadly expressed in multiple tissues. (A-I) Fluorescence from Prab-6.2::GFP in body wall muscles (A), pharyngeal muscle and head neurons (B), hypodermal cells (C), the seam cells (D), intestinal epithelia (E), coelomocytes (F), vulval epithelia (G), the distal tip cells (H), and the vulval muscles and ventral cord dendrites (I). (J-J'') Fluorescence from Prab-6.2::GFP (J) and Pglr-1::mRFP (J') with images merged (J''). Coexpressing neurons are outlined. Bars, 5 um.