Due to its simplicity, characteristic features, powerful genetics and complete genome sequence, C. elegans is an ideal model system to screen for flavonoids that are responsible for inducing growth inhibition and reducing brood size to develop natural nematocidal drugs. We examined several flavonoids including acacetin, apigenin, chrysin, daidzein, 5, 4-dihydroxyflavone, genkwanine, kaemferol, luteolin, naringenin, and quercetin by feeding C. elegans. Apigenin showed growth inhibition and induced reduction in brood size when it was treated at L1 stage. Effects of apigenin were accumulated and showed more severe growth inhibition in F1 generation than in P0 worms. Worms treated with apigenin also induced defective germ line development and showed reduction in brood size to 79.2 % of control that were grown without treatment in P0 worms and 37.2% in F1 generation. Worms treated with apigenin produced less number of germ cells than control in mitotic region. These results suggest that apigenin may inhibit cell division during the development of C. elegans. This study was supported by KRF2004-F00019 (KRF) and the second BK21 (MOE).

Consortium TCeGS (1994) Worm Breeder's Gazette "The C. elegans Genome Sequencing Project: A Progress Report."

Consortium TCeGS

[

Worm Breeder's Gazette,

1994]

The C. elegans genome sequencing project: A progress report. The C. elegans Genome Consortium, Genome Sequencing Center, Washington University School of Medicine, St. Louis, Missouri, USA and Sanger Centre, Hinxton Hall, Cambridge, UK.

Consortium TCeGS (1994) Worm Breeder's Gazette "The C. elegans Genome Sequencing Project: A Progress Report"

Consortium TCeGS

[

Worm Breeder's Gazette,

1994]

Abate JP et al. (2009) Cell Metab "Life is short, if sweet."

Blackwell TK, Abate JP

[

Cell Metab,

2009]

Insulin is essential for glucose homeostasis, but reducing its activity delays the aging process in model organisms. In this issue of Cell Metabolism, Lee et al. (2009) show how these effects of insulin signaling intersect when glucose is fed to C. elegans.

Mehra A et al. (1994) Worm Breeder's Gazette "Direct Interaction Between FEM-3 and a Carboxy-Terminal Fragment of TRA-2A"

Spence AM, Kuwabara PE, Mehra A, Heck L

[

Worm Breeder's Gazette,

1994]

Direct Interaction between FEM-3 and a Carboxy-Terminal Fragment Or TRA-2A Arun Mehra, Linda Heck, Patricia Kuwabara*, and Andrew Spence Dept. of Medical Genetics, University of Toronto, 1 King's College Circle, Toronto, Canada, and *MRC Laboratory of Molecular Biology, Hills Rd., Cambridge, UK

Lee C et al. (2017) Bio Protoc "Single-molecule RNA Fluorescence in situ Hybridization (smFISH) in Caenorhabditis elegans."

Lynch TR, Seidel HS, Lee C, Crittenden SL, Kimble J, Sorensen EB

[

Bio Protoc,

2017]

Single-molecule RNA fluorescence in situ hybridization (smFISH) is a technique to visualize individual RNA molecules using multiple fluorescently-labeled oligonucleotide probes specific to the target RNA ( Raj et al., 2008 ; Lee et al., 2016a ). We adapted this technique to visualize RNAs in the C. elegans whole adult worm or its germline, which enabled simultaneous recording of nascent transcripts at active transcription sites and mature mRNAs in the cytoplasm ( Lee et al., 2013 and 2016b). Here we describe each step of the smFISH procedure, reagents, and microscope settings optimized for C. elegans extruded gonads.

Weinkove D (2018) BMC Biol "On microbes, aging and the worm: an interview with David Weinkove."

Weinkove D

[

BMC Biol,

2018]

David Weinkove is an associate professor at Durham University, UK, studying host-microbe interactions in the model organism Caenorhabditis elegans. David has been focusing on the way microbes affect the physiology of their hosts, including the process of aging. In this interview, he discusses the questions shaping his research, how they evolved over the years, and his guiding principles for leading a lab.

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