Theresa Stiernagle (2006) WormBook "Maintenance of C. elegans."

Theresa Stiernagle

[WormBook, 2006]

Theresa Stiernagle et al. (2007) International Worm Meeting "Caenorhabditis Genetics Center."

Ann E Rougvie, Robert K Herman, Theresa Stiernagle

[International Worm Meeting, 2007]

The Caenorhabditis Genetics Center (CGC), supported by NIH NCRR, supplies Caenorhabditis strains and information to researchers throughout the world. On May 31, 2007, which marks the end of our current 15-year contract, Ann Rougvie will replace Bob Herman as CGC Director, and Bob will retain an advisory role as a Professor Emeritus. The CGC will continue to be housed at Minnesota and curated by Theresa Stiernagle. A five year renewal contract was submitted in August and is pending at NIH. We anticipate that a final (and positive) funding decision will be in hand by the start of the 2007 worm meeting. The CGC will continue its duties of acquiring, maintaining and distributing worm stocks, but other past activities of the CGC have been subsumed by WormBase. These include generating and maintaining a C. elegans bibliography, providing a clearinghouse for C. elegans genetic nomenclature, and maintaining a genetic map. The CGC now has over 8550 different strains. We strive to have at least one allele of every published gene and all chromosome rearrangements, duplications and deficiencies. In addition, we have several strains of species closely related to C. elegans. A searchable strains list will continue to be accessible either through the CGC website (www.cbs.umn.edu/CGC/) or through WormBase. Strains are available upon written request, which should include a brief statement of the intended use of the strains. Email requests (to cgc@umn.edu) are satisfactory. We provide quarterly reports to the NIH with statistics that reflect our services to the worm community. We like to be acknowledged in papers for providing strains. We also like to receive reprints of worm papers (.pdf files are encouraged), copies of which we also provide to NIH.

Theresa Stiernagle et al. (2003) International Worm Meeting "Caenorhabditis Genetics Center"

Robert Herman, Amy Daly, Leon Avery, Theresa Stiernagle, Chao-Kung Chen, Jonathan Hodgkin

[International Worm Meeting, 2003]

The Caenorhabditis Genetics Center (CGC), supported by NIH NCRR, supplies Caenorhabditis strains and information to researchers. The Minnesota team is responsible for acquiring, maintaining and distributing worm stocks, generating and maintaining a C. elegans bibliography, and publishing the Worm Breeder's Gazette (WBG). The English team acts as a clearinghouse for C. elegans genetic nomenclature and maintains the genetic map. The Texas team maintains the C. elegans web page. The CGC now has over 5000 different strains. We strive to have at least one allele of every published gene and all chromosome rearrangements, duplications and deficiencies. In addition, we have several strains of species closely related to C. elegans. Strains are available upon written request, which should include a brief statement of the intended use of the strains. Email requests (to stier@biosci.cbs.umn.edu) are satisfactory. The CGC bibliography currently includes over 5500 research articles, reviews and book chapters. The WBG is currently published twice each year. We would like comments about whether or not the WBG should be continued. Various types of information are available electronically from the CGC. You can get current strain lists, the C. elegans researcher directory, nomenclature guidelines, and the CGC bibliography at biosci.cbs.umn.edu/CGC. The English site contains a series of forms for submission of genetic map data, and this is the preferred method of data collection (www.sanger.ac.uk/Projects/C_elegans/CGC). Tables of data from the biennially produced map book are available for viewing or downloading. The Worm Breeder's Gazette and lots of other useful worm information are made available by Leon Avery at elegans.swmed.edu or at Nektarios Tavernarakis' European mirror of this site at elegans.imbb.forth.gr. We like to be acknowledged in papers for providing strains. We also like to receive reprints of worm papers (.pdf files are encouraged).

Theresa Stiernagle et al. (2005) International Worm Meeting "Caenorhabditis Genetics Center"

Leon Avery, Mary Ann Tuli, Robert Herman, Theresa Stiernagle, Jonathan Hodgkin, Amy Daly

[International Worm Meeting, 2005]

The Caenorhabditis Genetics Center (CGC), supported by NIH NCRR, supplies Caenorhabditis strains and information to researchers. The Minnesota team is responsible for acquiring, maintaining and distributing worm stocks, and generating and maintaining a C. elegans bibliography. The English team acts as a clearing house for C. elegans genetic nomenclature and maintains the genetic map. The Texas team maintains the C. elegans web page. The CGC now has over 6670 different strains. We strive to have at least one allele of every published gene and all chromosome rearrangements, duplications and deficiencies. In addition, we have several strains of species closely related to C. elegans. Strains are available upon written request, which should include a brief statement of the intended use of the strains. Email requests (to stier@cbs.umn.edu) are satisfactory. The CGC bibliography currently includes over 6870 research articles and book chapters. Various types of information are available electronically from the CGC. You can get current strain lists, the C. elegans researcher directory, nomenclature guidelines, and the CGC bibliography at biosci.cbs.umn.edu/CGC. The English site contains a series of forms for submission of genetic map data, and this is the preferred method of data collection (www.sanger.ac.uk/Projects/C_elegans/CGC). Tables of data from the biennially produced map book are available for viewing or downloading. The Worm Breeder's Gazette and lots of other useful worm information are made available by Leon Avery at elegans.swmed.edu or at Nektarios Tavernarakis European mirror of this site at elegans.imbb.forth.gr. We like to be acknowledged in papers for providing strains. We also like to receive reprints of worm papers (.pdf files are encouraged).

Theresa Stiernagle et al. (2001) International C. elegans Meeting "Caenorhabditis Genetics Center"

Leon Avery, Theresa Stiernagle, Robert Herman, Sylvia Martinelli, Jonathan Hodgkin

[International C. elegans Meeting, 2001]

Theresa Stiernagle et al. (1992) Worm Breeder's Gazette "The Caenorhabditis Genetics Center (CGC) Has Moved"

Theresa Stiernagle, Jonathan Hodgkin, Bob Herman

[Worm Breeder's Gazette, 1992]

On July 19, 1992 Mark Edgley arrived on the University of Minnesota campus in a rented truck containing frozen worms, computer and files. Mark stayed in St. Paul for two weeks to help us get organized and operational. We are extremely grateful to Mark and Don Riddle for the splendid way they have prepared for this transition. We shall try to continue the excellent tradition of CGC service they have established (pause for prolonged applause for Mark and Don). Jonathan has taken over responsibility for the genetic map. Genetic map data for new genes, improved locations for known genes, and new data on rearrangements (duplications, deficiencies, balancers, and so on) should all be sent Cambridge UK (or St. Paul, MN for forwarding to Cambridge), using standard formats. Forms are available on request. In order to improve the correlation between the genetic and physical maps, the CGC also wishes to collect published and unpublished genetic map data for genes, sequences and RFLP's with defined positions on the physical map. Data should be sent to Cambridge UK or St. Paul, MN, using (as far as possible) the standard genetic map data formats, as above. We also encourage submission of genetic map data by e-mail. Investigators who wish to use this option should send a message to jah@mrc-lmb.cam.ac.uk. What follows are reminders of additional ways in which you can help us: 1. Please try to remember to acknowledge in papers the use of any strains received from the CGC. 2. Please send us reprints of au of your papers; if a paper acknowledges the CGC, we would be happy to receive two reprints, because we must send one to NIH. 3. Please let us know if you find a bibliographic reference we have missed (particularly if it is one of your own). 4. Our first priority in acquiring strains is to acquire a reference allele of every identified gene and all available chromosome rearrangements. If you can help us fill gaps in our collection without our asking, all the better. 5. We need your strain requests in writing (e-mail is fine), with a brief statement of research or training activity for which the strains are intended.

Rougvie, Ann E. et al. (2009) International Worm Meeting "Caenorhabditis Genetics Center."

Stiernagle, Theresa, Rougvie, Ann E.

[International Worm Meeting, 2009]

The Caenorhabditis Genetics Center (CGC), supported by the National Institutes of Health - National Center for Research Resources (NIH-NCRR), supplies Caenorhabditis strains and information to researchers throughout the world. The CGC continues to be housed at the University of Minnesota, but will see changes in the next year as Theresa Stiernagle retires as Curator and pursues other interests. The CGC will continue its duties of acquiring, maintaining and distributing worm stocks. The CGC now has over 11,499 different strains. We strive to have at least one allele of every published gene and all chromosome rearrangements, duplications and deficiencies. Selected multiple-mutant stocks and transgenic strains are also available including strains that express various green fluorescent protein (GFP) reporter fusions. The CGC also has stocks of nematode species closely related to C. elegans and bacterial strains necessary for nematode growth. A searchable strains list, including information about CGC stocks, is accessible either through the CGC website (www.cbs.umn.edu/CGC/) or through WormBase. Requests for strains should be made via the on-line ordering system available through our website. As mandated by NIH-NCRR, a small yearly user fee and charge per strain is assessed with each order. The CGC strongly encourages use of credit cards for these charges. We provide quarterly reports to the NIH with statistics that reflect our services to the worm community. We especially like to be acknowledged in papers for providing strains. We also like to receive pdf files of such papers, copies of which we provide to NIH.

Leon Avery et al. (2002) Worm Breeder's Gazette "C. elegans Researcher Directory"

Leon Avery, Theresa Stiernagle, Bob Herman

[Worm Breeder's Gazette, 2002]

Ewbank JJ et al. (1998) Worm Breeder's Gazette "600 million years of Phox2."

Brunet J-F, Pattyn A, Ewbank JJ

[Worm Breeder's Gazette, 1998]

We are studying a C. elegans homologue of a couple of vertebrate neuron-specific transcription factors, to explore phylogenetic aspects of neuronal identity and of its transcriptional determination. Phox2a and Phox2b are two closely related homeodomain proteins of the paired-like family specifically expressed (mostly co-expressed) in specific classes of neurons in vertebrates (1,2). Careful analysis of their expression pattern has revealed a striking coherence which can be summarized by three overlapping neuroanatomical correlates: i) all noradrenergic neurons, ii) most central and peripheral neurons involved in the reflex circuits of the visceral nervous system, iii) cranial motoneurons. Published work and ongoing studies aim at reconstructing the transcriptional cascades in which Phox2a and Phox2b act and at defining which part of specific neuronal phenotypes they control (3,4). The sequencing of the C. elegans genome has unveiled a probable orthologue of Phox2a and b, D1007.1, which we call for the moment cePhox2. It is 88% identical to murine Phox2a in the homeodomain, a level of homology often associated with functional conservation among homeobox genes between vertebrates and invertebrates. We have cloned the cDNA by RT-PCR, thereby verifying the GeneFinder prediction. We have created transgenic lines harboring GFP under the control of the 1 kb upstream of CePhox2. The transgene is first expressed in early embryos. Expression continues at larval and adult stages in a few head and tail neurons, and, presumably ectopically, in the rostral and caudal part of the intestine. An antibody raised against the C-terminus of CePhox2 has confirmed its neuronal expression. Our current aim is to identify each Phox2 expressing neuron. We then plan to perform loss-of-fuction and gain-of-function studies. Our long-term hope, with the parallel mouse and worm studies, is to define what has changed and what has not throughout evolution in the "neuro-ontogenetic module" involving Phox2. Thanks to: Alan Coulson, Andy Fire and Theresa Stiernagle. (1) Tiveron et al. (1996) J. Neurosci., 16, 7649-7660. (2) Pattyn et al. (1997) Development, 124, 4065-4075. (3) Morin et al. (1997) Neuron, 18, 411-423. (4) Hirsch et al. (1998) Development, 125, 599-608.

Mary-Ann Tuli et al. (2007) International Worm Meeting "CGC and WormBase."

Jonathan Hodgkin, Mary-Ann Tuli, Theresa Stiernagle, Robert Herman, Richard Durbin

[International Worm Meeting, 2007]

WormBase is an international consortium of biologists and computer scientists from Caltech (USA), Cold Spring Harbour Laboratory (USA), Wellcome Trust Sanger Institute (UK) and the Genome Sequencing Center at Washington University (USA). The UK WormBase group works closely with the Caenorhabditis Genetics Center (CGC) to curate genetic nomenclature and maintain the C.elegans genetic map. Detailed guidelines are accessible via http://www.cbs.umn.edu/CGC/Nomenclature/nomenguid.htm Release WS170 (Jan 2007) contains 54213 WBGeneIDs (unique gene identifiers) of which 25533 are from C.elegans and 19666 from C.briggsae. The number of CDS objects which have been assigned a CGC approved gene name in release WS170 (Jan 2007) is 7300 (compared to 5707 in release WS140 (March 2005). Strain data is sent to WormBase from the CGC in Minneapolis every month. The extensive cross referencing within these objects allows users to easily find information relating to a specific strain. The number of strains in WS170 is 13000 of which 8000 are stored at the CGC. Manual curation of allele data by WormBase biologists is an ongoing project and recent efforts have seen the backlog of post-2001 publications cleared. Many of these alleles have details of the strain carrying the mutation and a description of the lesion. If we know the location of a mutation, we are now able to automatically attach an accurate description of how it affects a gene and give the amino acid change if applicable. This has considerably enhanced allele representation in WormBase. We are now able to start working on pre-2001 publications. Allele information is either captured from scientific journals or submitted directly by researchers using the WormBase submission forms at http://wormbase.org/db/curate/online_forms We continue our long term collaboration with the C. elegans Gene Knockout Consortium ( http://celeganskoconsortium.omrf.org/ ) and the National Bioresource Deletion Mutant Project ( http://shigen.lab.nig.ac.jp/c.elegans/index.jsp ) A link to the source database from WormBase allows users to view more detailed information about individual alleles. Since WS153 (Dec 2005) WormBase has been collaborating with the NemaGENETAG Mos insertion project ( http://elegans.imbb.forth.gr/nemagenetag/ ) and in WS174 had over 4000 alleles including full details of the Mos insertion site. We have developed a web form where collaborators can upload their data which has streamlined the submission procedure. WormBase is supported by a grant from the National Human Genomes Reseach Institute at the US National Institute of Health #P41 HG02223 and the British Medical Research Council. CGC is supported by NIH NCRR.