[
Neuron,
2001]
Weighing in at about 5 ug, with 302 neurons and 5000 synapses, C. elegans is unlikely to prove theorems, write poetry, or challenge Mike Tyson. Still, remarkable behavioral complexity is packed into this tiny worm.
[
J Fungi (Basel),
2018]
<i>C. elegans</i> has several advantages as an experimental host for the study of infectious diseases. Worms are easily maintained and propagated on bacterial lawns. The worms can be frozen for long term storage and still maintain viability years later. Their short generation time and large brood size of thousands of worms grown on a single petri dish, makes it relatively easy to maintain at a low cost. The typical wild type adult worm grows to approximately 1.5 mm in length and are transparent, allowing for the identification of several internal organs using an affordable dissecting microscope. A large collection of loss of function mutant strains are readily available from the <i>C. elegans</i> genetic stock center, making targeted genetic studies in the nematode possible. Here we describe ways in which this facile model host has been used to study <i>Candida albicans</i>, an opportunistic fungal pathogen that poses a serious public health threat.
[
J Invertebr Pathol,
2024]
The free-living nematode Pristionchus pacificus has been established as a model system in integrative evolutionary biology by combining laboratory studies with field work and evolutionary biology. Multiple genetic, molecular and experimental tools and a collection of more than 2,500P. pacificus strains and more than 50 Pristionchus species, which are available as living cultures or frozen stock collections, support research on various life history traits. Species of Pristionchus exhibit a number of complex traits unknown from Caenorhabditis elegans and most other free-living nematodes. First, P. pacificus can form two alternative mouth forms, an example of developmental plasticity that is increasingly studied to investigate the role of plasticity as a facilitator of evolutionary novelty. More than a decade of work has identified associated genetic and epigenetic mechanisms and revealed the evolutionary and ecological significance of feeding structure plasticity. Second, one of the two mouth morphs results in predatory behavior against other nematodes and is currently used to investigate the neurobiology of predation. Third, potential predation results in the risk of cannibalism among conspecifics. Strikingly, Pristionchus nematodes have developed a self-recognition system that allows the distinction of self (kin) and non-self. Given all these organismal features, this nematode has recently been considered a key example for research towards a new natural history (West-Eberhard, 2024). Here, I summarize recent work on Pristionchus with a focus on a 'new natural history'. In addition, I review some recent studies that indicate an interaction of Pristionchus with EPNs that was suggested based on various surveys in different ecological habitats.