-
[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of hydrogen peroxide in water solutions on the nematode life span. In this experiment hydrogen peroxide was used in following dilutions: 100 mg/L, 10 mg/L, 1 mg/L, 0.1 mg/L, 0.01 mg/L and 0.001 mg/L. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without hydrogen peroxide) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without hydrogen peroxide in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with hydrogen peroxide in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Conclusion: If hydrogen peroxide solution was applied to C. elegans, it was not able to increase their mean longevity significantly in comparison with control.
-
[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of glycerol in water solutions on the nematode life span. In this experiment glycerol was used in following dilutions: 100 g/L, 10 g/L, 1 g/L, 100 mg/L, 10 mg/L, 1 mg/L and 0.1 mg/L. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without glycerol) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without glycerol in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with glycerol in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.
-
[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of kudesan (a water-soluble medicine, containing 30.0 mg of ubiquinone and 4.5 mg of a-tocopherol in 1 mL) in water solutions on the nematode life span. In this experiment kudesan was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without kudesan) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without kudesan in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with kudesan in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.
-
[
Worm Breeder's Gazette,
1980]
Cyclic AMP levels have been assayed in N2 synchronous cultures and starvation-induced dauer larvae as a first step in the analysis of hormonal involvement in dauer larva formation, maintenance, and recovery. N2 worms are synchronized by egg purification (after the method of Ralph Hecht), hatching into M9 buffer, and resuspension in liquid worm medium containing an adenyl cylase deletion mutant of E. coli. The worms are harvested at various time intervals by immediately microwaving to boiling and washing in glass distilled water. This is followed by lyophillization after which the dry weight is determined. The assay uses a cAMP binding protein (Calbiochem) in a competitive binding assay with [3H]cAMP. After equilibrium is reached, the protein is filtered through a nitrocellulose filter and counted in a liquid scintillation counter. Initial results indicate that changes in cAMP concentration are correlated with the life cycle. Assays performed on well fed worms at various times in larval development show a linear decrease in cAMP levels from 25 pmol/mg dry weight in L2's to 15 pmol/mg dry weight in L3's, down to 1 pmol/mg dry weight in L4's. Starvation-induced dauer larvae have a cAMP level of 5 pmol/mg dry weight, or about one-third the level present at the L2 molt. Recovering dauers isolated 6 hours after the addition of food to starvation-induced dauers, show a dramatic drop in cAMP levels to less than 0.1 pmol/mg dry weight. The data collected to this point indicate that dauer larvae have a reduced level of cAMP compared to the L2 an' L3 larval stages and that recovery from the dauer stage results in cAMP levels below that found in L4's. These preliminary studies are to be followed by a more detailed study of cAMP and cGMP levels in N2 worms at all stages of the life cycle and in selected dauer-constitutive mutants. Cyclic GMP assays and pharmacological studies using theophylline as a phosphodiesterase inhibitor are currently being developed.
-
[
Worm Breeder's Gazette,
1976]
RNA synthesis has been studied during oogenesis by autoradiography. Allow incorporation to occur, ovary has been dissected out of gravid adults into Mg medium containing 3H uridine. RNA is synthesized actively until the end of meiotic prophase. This synthesis follows a classical model : label appears at first in the nucleus and after in the cytoplasm. This 'in vitro system' gives homogeneous results in each sample .
-
[
Worm Breeder's Gazette,
2002]
The purpose of this study was to investigate the effect of acetylsalicylic acid in water solutions on the nematode life span. In this experiment acetylsalicylic acid was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without acetylsalicylic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without acetylsalicylic acid in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with acetylsalicylic acid in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Conclusion: If acetylsalicylic acid solution was applied to C. elegans in concentration of 10 mg/L, it was able to increase significantly (P>0.05) their mean longevity in comparison with control to 83.96 percent.
-
[
Worm Breeder's Gazette,
1980]
By maintaining C. elegans on ampicillin treated E. coli a high throughput inexpensive screen for detecting potential anthelmintics is obtained. Method: 5 mg of ampicillin and 1.7 mg nystatin are added to 10 mls of E. coli broth (Uracil requiring mutant) and 100 mls of M9 buffer and the mixture stood for 2 hrs. Multi well plates are filled with 2 mls of the mixture and substances to be tested are added in the minimum volume of water, ethanol or DMSO. Between 12 and 20 worms of mixed age are added per well and after 7 days incubation at 20 C experimental wells are compared to controls for numbers of larvae and adults and normal movement. Most benzimidazoles are detected at 1 p.p.m. with the exceptions of oxfendazole (10 p.p.m.) and thiabendazole (2.5 p.p.m.). Levamisole is detected down to 0.5 p.p.m. and avermectin Bla to 0.0005 p.p.m. At 50 p.p.m. less than 10% of random compounds are active. Although older drugs such as piperazine , bephenium, diethylcarbamazine, methyridine and santonin would be missed this is not considered to be of significance as modern drugs must be both broadspectrum and very active. The test can also probably be used to detect potential plant nematocides.
-
[
Worm Breeder's Gazette,
2002]
The purpose of this study was to investigate the effect of streptomycin sulphate in water solutions on the nematode life span. In this experiment streptomycin sulphate was used in following dilutions: 1.0 and 0.1 mg/ml. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without streptomycin sulphate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without streptomycin sulphate in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3th day, these worms were transferred every day in next wells containing medium with streptomycin sulphate in any concentration. This investigation was carried out in temperature +21C and in the darkness.
-
[
Worm Breeder's Gazette,
1975]
E1392 (
nuc-1,X) lacks the principal endodeoxyribonuclease. When determined on crude sonicates by an assay sensitive to endolytic cleavage of DNA (e.g. the spectrophotometric method of Kunitz, reviewed in Prog. in Nucl. Acid Res. 1 85 (ed Cantoni and Davies, 1967)), the DNase activity is well under 1% of that in N2. Feulgen staining reveals stranded material in the gut, persistent DNA from programmed cell deaths, and extruded polar bodies accumulating in the gonads of old animals; this suggests that a single endo-DNase is used both in the gut and in the tissues of C. elegans. There is no visible phenotype. The map position is: [See Figure 1] The endo-DNase of N2 is equally active in the presence of Mg++ and of EDTA
-
[
Worm Breeder's Gazette,
1992]
After tabulating the results of the Worm Plate Survey. we have come up with some interesting results. Most notably. the high variability in prices that labs are paying for their plates, even for the exact same plates from the same supplier, and the fact that most plates are marked up considerably over the actual cost. The replies can be separated into 4 categories: Labs that get plates from Fisher ($29-$58). but wish they had non-vented plates Labs that get non-vented plates via Applied Scientific (~$38) Labs that get plates from Falcon (vented) or Nunc (non-vented) and pay much more Most labs' plates were "slipable" or "semi-stackable", but all labs wanted plates that stack well for easy manual pouring, seeding, carrying, and using. Everyone wanted plates with shallow lids such that the bottoms can be lifted out of the tops for inverted use. Some labs expressed an interest in plates slightly smaller than "60 mm". That number is in quotes because all of the companies' plates have bottoms smaller than 60 mm (e.g. Fisher -54 x 14 mm). We have negotiated with the plastic companies that really make the plates for Fisher, Applied Scientific, etc. (that actually just resell them to you). I have come to the conclusion that we can provide you with better worm plates, the same worm plates cheaper, or in most cases better worm plates cheaper. This is true for every lab. The bottom line is that we can get you top quality non-vented "60 mm" plates (like Applied Scientific's, except fully stackable) for about $29 per 500 case INCLUDING shipping depending on your usage and how many cases you can receive at one time. Several labs have found the non-vented plates last longer without drying out or getting contaminated, compared with normal vented plates, so you should save that way, too. We offer full service shipping (e.g. standing orders and same-day telephone orders, free. Similarly low prices are available on 100 mm and 150 mm plates that exceed industry standards for flatness (reducing media usage) and clarity. The 100 mm are about $27 per 500 case plus shipping; The 150 mm dishes (good for DNA & RNA preps and library platings, with more than 2.25x the surface area of 100 mm dishes) are made thicker and deeper than industry standards and are about $21.50 per 100 case plus shipping. The shipping charge is very low for labs, or groups of labs in one city, that can take delivery of many cases in a single shipment. You can even suggest that your stockroom order plates from us. Call us for an exact price quote depending on your usage and how many cases you can receive at one time. In any case, we'll work things out to save you money. In the future, we can offer inexpensive 35 mm dishes if the community at large can order about 2000 cases per year, so let me know about your needs for other sizes. The response was very mixed about pre-poured plates. We may set that up later, but for now we can help the most by saving you lots on empty petri dishes (and later, maybe media .supplies). We are happy to send out free samples so you can examine the dishes. If we haven't contacted you yet, just give us a call. Respondents: 38 (including 5 anonymous) "Winners": Horvitz = 550, Meyer = 400, Thomas = 400, Greenwald = 300 200-299 cases 8 labs 100-199 cases 7 labs 4-99 cases 19 labs Highest price per case: US = 118.75, Canada = $117 (non-vented) Lowest price per case: US = $29, Canada = $25 (vented) Farthest away response: Malta! No responses from MRC or anyone else in Europe or Asia. It is possible that we can save money and/or provide better plates for these labs, including, shipping, too. Let us know.