Ciche T (2007) WormBook "The biology and genome of Heterorhabditis bacteriophora."

Ciche T

[WormBook, 2007]

Heterorhabditis bacteriophora is an entomopathogenic nematode (EPN) mutually associated with the enteric bacterium, Photorhabdus luminescens, used globally for the biological control of insects. Much of the previous research concerning H. bacteriophora has dealt with applied aspects related to biological control. However, H. bacteriophora is an excellent model to investigate fundamental processes such as parasitism and mutualism in addition to its comparative value to Caenorhabditis elegans. In June 2005, H. bacteriophora was targeted by NHGRI for a high quality genome sequence. This chapter summarizes the biology of H. bacteriophora in common and distinct from C. elegans, as well as the status of the genome project.

Freeman MN et al. (1998) "Establishment of quality assurance procedures for aquatic toxicity testing with the nematode Caenorhabditis elegans"

Freeman MN, Williams PL, Marse TJ

[1998]

In this study initial data were generated to develop laboratory control charts for aquatic toxicity testing using the nematode Caenorhabditis elegans. Tests were performed using two reference toxicants: CdCl2 and CuCl2. All tests were performed for 24 h without a food source and for 48 h with a food source in a commonly used nematode aquatic medium. Each test was replicated 6 times with each replicate having 6 wells per concentration with 10 +/- 1 worms per well. Probit analysis was used to estimate LC50 values for each test. The data were used to construct a mean laboratory control chart for each reference toxicant. The coefficient of variation (CV) for three of the four reference toxicant tests was less than 20%, which demonstrates an excellent degree of reproducibility. These CV values are well within suggested standards for determination of organism sensitivity and overall test system credibility. A standardized procedure for performing 24 h and 48 h aquatic toxicity studies with C. elegans is

Kam N et al. (2004) Modeling in Molecular Biology. G Ciobanu and G Rozenberg (eds). Natural Computing Series, Springer-Verlag. "Formal modeling of C. elegans development: a scenario-based approach."

Pnueli A, Kam N, Hubbard EJA, Kugler H, Marelly R, Stern MJ, Harel D

[Modeling in Molecular Biology. G Ciobanu and G Rozenberg (eds). Natural Computing Series, Springer-Verlag., 2004]

We present preliminary results of a new approach to the formal modeling of biological phenomena. The approach stems from the conceptual compatibility of the methods and logic of data collection and analysis in the field of developmental genetics with the languages, methods and tools of scenario-based reactive system design. In particular, we use the recently developed methodology consisting of the language of live sequence charts with the play-in/play-out process, to model the well-characterized process of the cell fate acquisition during C. elegans