Plenefisch JD et al. (1994) Worm Breeder's Gazette "Cloning mua-3: Some Observations on the New Molecular Era"

Hedgecock EM, Plenefisch JD

[Worm Breeder's Gazette, 1994]

Cloning mua-3: some observations on the new Molecular Era John Plenefisch and Edward Hedgecock, Dept. of Biology, Johns Hopkins University, Baltimore MD 21218

Hu KJ et al. (1999) Nematology "Nematicidal metabolites produced by Photorhabdus luminescens (Enterobacteriaceae), bacterial symbiont of entomopathogenic nematodes."

Webster JM, Hu KJ, Li JX

[Nematology, 1999]

The secondary metabolites, 3,5-dihydroxy-4-isopropylstilbene (ST) and indole, from the culture filtrate of Photorhabdus luminescens MD, were shown to have nematicidal properties. ST caused nearly 100% mortality of 54 and adults of Aphelenchoides rhytium, Bursaphelenchus spp. and Caenorhabditis elegans at 100 mu g/ml, but had no effect on J2 of Meloidogyne incognita or infective juveniles (IJ) of Heterorhabditis megidis at 200 mu g/ml. Indole was lethal to several nematode species at 300 mu g/ml, and caused a high percentage of Bursaphelenchus spp. (54 and adults), M, incognita (J2) and Heterorhabditis spp. (IJ) to be paralysed at 300, 100 and 400 mu g/ml, respectively. Both ST and indole inhibited egg hatch of M, incognita. ST repelled IJ of some Steinernema spp. but not IJ of Heterorhabditis spp., and indole repelled IJ of some species of both Steinernema and Heterorhabditis. ST, but not indole, was produced in nematode-infected larval Galleria mellonella. after 24 h infection.

Hresko MC et al. (1994) Worm Breeder's Gazette "Shoot First, Ask Questions Later"

Shrimankar PV, Waterston RH, Hresko MC

[Worm Breeder's Gazette, 1994]

Shoot First, Ask Questions Later M.C Hresko, P.V. Shrimankar and R.H. Waterston. Washington Univ. Sch. of Med., St. Louis, MO 63110. coutu@sequencer.wustl.edu and pvs@elegans.wustl.edu

Eddy SR "The Caenorhabditis elegans genome project."

Eddy SR

In the next five years, molecular biology will get its first look at the complete genetic code of a multicellular animal. The Caenorhabditis elegans genome sequencing project, a collaboration between Robert Waterston's group in St. Louis and John Sulston's group in Cambridge, is currently on schedule towards its goal of obtaining the complete sequence of this organism and all its estimated 15,000 to 20,000 genes by 1998. By that time, we should also know the complete genome sequence of a few other organisms as well, including the prokaryote Escherichia coli and the single-celled eukaryote Saccharomyces

Parham C et al. (1994) Worm Breeder's Gazette "Tc4 and Tc5: What Makes Them Move and Why It Matters"

Collins JJ, Butze K, Parham C, Beinhorn J

[Worm Breeder's Gazette, 1994]

Tc4 and Tc5: what makes them move and why it matters Christi Parham, Kristie Butze, Joanna Beinhorn and John Collins. Dept. of Biochemistry and Molecular Biology, University of New Hampshire. Durham, NH 03824

Laura Gallo et al. (2003) International Worm Meeting "A genetic approach to identify meiotic-specific chromosome segregation factors"

Kathleen St. John, Laura Gallo, Erica Stewart, Diane C Shakes, Ken Donohue

[International Worm Meeting, 2003]

During the normal process of eukaryotic cell division, chromosomes replicate during S-phase, undergo ordered compaction during prophase, and align in a central plate during metaphase. As cells subsequently transition from metaphase to anaphase, the replicated and aligned chromosomes separate and segregate into each of the two daughter cells. During anaphase, defects in any step of this multi-step process result in chromosome breakage and/or mis-segregation, problems that ultimately result in birth defects, cell death, and/or malignant transformations. Recent studies in a variety of eukaryotes suggest that the basic molecular mechanisms of chromosome segregation have been conserved both across species and between the related but distinctive processes of mitosis and meiosis. At the same time, the meiotic-specific process of homolog separation requires at least a few meiotic specific components, and chromosome segregation on anastral, acentriolar spindles is similarly expected to require at least a few oocyte-specific components. To identify the combination of unique and cell-type specific genes required for proper anaphase chromosome segregation in C. elegans, we are screening through a large collection of temperature-sensitive maternal mutants originally isolated by Matt Wallenfang and Geraldine Seydoux. Our overall strategy is to identify those with obvious defects in oocyte meiotic chromosome segregation and then to secondarily determine which of these also have defects in spermatocyte meiosis and/or germline mitosis. Using this approach, we expect to not only identify genes required during different stages of chromosome segregation but also those that differ in their cell-type specificity including those with general (mitosis and meiosis); meiosis-specific; and oocyte-specific defects. To date, subclasses of oocyte defects include problems in meiotic metaphase alignment, homolog separation, and meiotic cell cycle progression. Genetic mapping studies of these mutants as well as a comparative analysis of their oocyte, spermatocyte, and germline defects are currently in progress.

McCarter JP et al. (1994) Worm Breeder's Gazette "Somatic Regulation of Germ-line Development. Introduction and Part I; Mitotic Proliferation"

McCarter JP, Schedl TB

[Worm Breeder's Gazette, 1994]

Somatic Regulation of Germ-line Development Introduction, and Part I; Mitotic Proliferation Jim McCarter and Tim Schedl. Dept. of Genetics, Washington Univ. School of Medicine, St. Louis, MO 63110, jim@wugenmail.wustl.edu

Consortium TCeGS (1994) Worm Breeder's Gazette "The C. elegans Genome Sequencing Project: A Progress Report."

Consortium TCeGS

[Worm Breeder's Gazette, 1994]

The C. elegans genome sequencing project: A progress report. The C. elegans Genome Consortium, Genome Sequencing Center, Washington University School of Medicine, St. Louis, Missouri, USA and Sanger Centre, Hinxton Hall, Cambridge, UK.

Li WQ et al. (1992) Worm Breeder's Gazette "Characterization of the Axonal Guidance and Outgrowth gene Unc-33"

Herman RK, Shaw JE, Li WQ

[Worm Breeder's Gazette, 1992]

Characterization of the axonal guidance and outgrowth gene unc-33 W. Li, R. K. Herman and J. E. Shaw Department of Genetics and Cell biology, University of Minnesota, St Paul, MN 55108

Consortium TCeGS (1994) Worm Breeder's Gazette "The C. elegans Genome Sequencing Project: A Progress Report"

Consortium TCeGS

[Worm Breeder's Gazette, 1994]

The C. elegans genome sequencing project: A progress report. The C. elegans Genome Consortium, Genome Sequencing Center, Washington University School of Medicine, St. Louis, Missouri, USA and Sanger Centre, Hinxton Hall, Cambridge, UK.