To determine if AGEF-1 regulates LET-23 EGFR localization, authors compared the ratio of basolateral versus apical localization of LET-23::GFP in the P6.p cell of wild-type and
agef-1(
vh4) animals. In wild-type, the average basal/apical intensity of LET-23::GFP in P6.p was 0.49 for zhIs035 and 0.65 for zhIs038. In
agef-1(
vh4) animals, the average basal/apical intensity of LET-23::GFP in the P6.p cell is 0.79 for zhIs035 and 0.93 for zhIs038 reflecting a decrease in apical intensity and an increase in basolateral intensity. We also found LET-23::GFP is present on the basolateral membrane of the intestinal cells in agef- 1
(vh4) animals whereas we did not see this in wild-type by confocal microscopy. Therefore, AGEF-1 repress- es basolateral localization of LET-23::GFP in the VPCs and intestinal cells.