In most control animals, there were 12 VU cells expressing lin-11::gfp. In egl-43i animals, the number of lin-11::gfp-positive cells was reduced to fewer than four gfp-expressing VU cells.
In most control animals, there were 12 VU cells expressing egl-13::gfp. In egl-43i animals, the number of egl-13::gfp-positive cells was reduced to fewer than four gfp-expressing VU cells.
In fos-1(ar105) homozygous L3 larvae carrying the 1.7-Lp7gfp reporter, no GFP expression could be detected in the AC, and GFP expression in the VU cells was also strongly reduced.
Suppression of the anchor cell deficient phenotype observed in activated LIN-12 (lin-12(n137)), Thus, egl-43 acts downstream of or in parallel to lin-12 during AC/VU specification.
fos-1 RNAi eliminated egl-43::YFP expression in the DU and VU descendants as well as in the AC, suggesting that fos-1 is an upstream regulator of egl-43 in these gonadal cells.