"...We measured gene induction during exposure to live S. aureus, which causes pathogenesis, or heat-killed S. aureus, which does not (Figures 3G-I and S4C, E). Unexpectedly, all 10 biomarker genes were induced at least equally well on heat-killed S. aureus as on live S. aureus (Figure 6A)."
"In wild-type C. elegans embryos, AIR-2 localizes to the kinetochores of chromosomes in early mitosis, and to the central spindle and midbody in anaphase and telophase (Schumacher et al., 1998). In ZEN-4-depleted embryos, which lack dense microtubule bundles between the chromosome masses, AIR-2 localization is restricted to small microtubule bundles (Powers et al., 1998). Similarly, we observed that some microtubules were still labeled by anti-AIR-2 antibodies in CeCDC-14-depleted embryos (n = 5), even though no compact central spindles were assembled (Fig. 4 B, bottom)."
Query-target gene pairs were tested for interaction by feeding target gene RNAi to worms with a mutation in the query gene. Each query-target pair and its two controls were tested in at least three rounds.|Target genes were classified as either belonging to LGIII (L) or to signalling pathways (S) or to both (L,S): S