"To rule out the possibility that high temperature (25°C) could influence the outcome by modulating expression of UPRmt genes independently of the mitochondrial stress, we performed subsequent experiments at 20°C in addition to 25°C. Once again, deletion of
atfs-1 failed to significantly attenuate lifespan extension from
cco-1(RNAi) at both temperatures (Fig. 5c,d)."