fog-2 : sacy-1

Feminization with strong loss-of-function mutations in the sex determination pathway delayed the time of onset and the severity of oocyte degeneration in sacy-1(tm5503) females (Figure 8 and Figure S3; S. Kim and D. Greenstein, unpublished results).

che-3 : osm-6

"osm-6::gfp is expressed in ciliated neurons... animals mutant for che-3, which encodes a dynein heavy chain (W. Grant, personal communication), showed enhanced GFP accumulation in two large bilateral patches near the region of the dendritic endings of the amphid neurons (Figure 6C) and also near the phasmid endings."

hsp-16.1 : microwave exposure (20hrs)

"Table 1 shows a typical pattern of heat-shock reporter induction in adult PC72 C. elegans. Relative to the minimal baseline expression levels at 15C, there is a modest 5-fold increase in expression in negative controls at 25C. This is increased by a further 3-4 fold after 20 h of exposure either at 28C or to weak microwave fields (1 GHz and 0.5 W) at 25C, giving a net 15-20 fold increase over baseline expression at 15C [1]."

heat treatment (28C for 20hrs) : hsp-16.1

"Table 1 shows a typical pattern of heat-shock reporter induction in adult PC72 C. elegans. Relative to the minimal baseline expression levels at 15C, there is a modest 5-fold increase in expression in negative controls at 25C. This is increased by a further 3-4 fold after 20 h of exposure either at 28C or to weak microwave fields (1 GHz and 0.5 W) at 25C, giving a net 15-20 fold increase over baseline expression at 15C [1]."

fog-1 : sacy-1

Feminization with strong loss-of-function mutations in the sex determination pathway delayed the time of onset and the severity of oocyte degeneration in sacy-1(tm5503) females (Figure 8 and Figure S3; S. Kim and D. Greenstein, unpublished results). In all female backgrounds tested,we observed oocytes in sacy-1(tm5503) females to undergo meiotic maturation and ovulation at apparently high rates;the uterus filled with unfertilized oocytes (Figure 8A and Figure S3C). We also observed apparently defective ovulation in sacy-1(tm5503) females such that the gonad arms often contained endomitotic oocytes. We confirmed that MSP was undetectable in sacy-1(tm5503) females as expected (Figure S6).

Cbr-bar-1 : egl-18 : elt-6

"240 genes that were upregulated in the Wnt-activated samples compared with the Wnt-inhibited samples were identified (L.G., W. Chen, T. Brodigan, M. Krause and D.M.E., unpublished). Among these potential Wnt pathway targets, were the GATA factor genes egl-18 and elt-6 [average fold change of 2.1 (P=0.06) and 2.8 (P=0.01), respectively]. We confirmed the microarray result by qRT-PCR on animals overexpressing stabilized β-catenin BAR-1 at the L2/L3 molt (supplementary material Fig. S2)."

fem-3 : sacy-1

Feminization with strong loss-of-function mutations in the sex determination pathway delayed the time of onset and the severity of oocyte degeneration in sacy-1(tm5503) females (Figure 8 and Figure S3; S. Kim and D. Greenstein, unpublished results). In all female backgrounds tested,we observed oocytes in sacy-1(tm5503) females to undergo meiotic maturation and ovulation at apparently high rates;the uterus filled with unfertilized oocytes (Figure 8A and Figure S3C). We also observed apparently defective ovulation in sacy-1(tm5503) females such that the gonad arms often contained endomitotic oocytes. We confirmed that MSP was undetectable in sacy-1(tm5503) females as expected (Figure S6).

fog-3 : sacy-1

Feminization with strong loss-of-function mutations in the sex determination pathway delayed the time of onset and the severity of oocyte degeneration in sacy-1(tm5503) females (Figure 8 and Figure S3; S. Kim and D. Greenstein, unpublished results). In all female backgrounds tested, we observed oocytes in sacy-1(tm5503) females to undergo meiotic maturation and ovulation at apparently high rates; the uterus filled with unfertilized oocytes (Figure 8A and Figure S3C). We also observed apparently defective ovulation in sacy-1(tm5503) females such that the gonad arms often contained endomitotic oocytes. We confirmed that MSP was undetectable in sacy-1(tm5503) females as expected (Figure S6).

bar-1 : pry-1

"Epistasis analysis was performed with pry-1(mu38) and the mutations bar-1(ga80) and mig-14(ga62), which cause too few VPCs to adopt induced fates. The ga80 mutation introduces an early stop codon in BAR-1, and is predicted to cause a null mutant phenotype (Eisenmann et al. 1998). mig-14(ga62) is a viable, reduction-of-function mutation, which causes defects in multiple Wnt-mediated processes in embryogenesis and post-embryonic life (Eisenmann and Kim 2000). The Overinduced phenotype of pry-1(mu38) is still manifest in a double mutant with mig-14(ga62), but is strongly reduced in a double mutant with bar-1(ga80) (Table 1)." With regard to vulval cell induction, pry-1(mu38) is epistatic to mig-14(ga62) and bar-1(ga80) is epistatic to pry-1(mu38).

R06B9.7 : mig-14 : pry-1

"Epistasis analysis was performed with pry-1(mu38) and the mutations bar-1(ga80) and mig-14(ga62), which cause too few VPCs to adopt induced fates. The ga80 mutation introduces an early stop codon in BAR-1, and is predicted to cause a null mutant phenotype (Eisenmann et al. 1998). mig-14(ga62) is a viable, reduction-of-function mutation, which causes defects in multiple Wnt-mediated processes in embryogenesis and post-embryonic life (Eisenmann and Kim 2000). The Overinduced phenotype of pry-1(mu38) is still manifest in a double mutant with mig-14(ga62), but is strongly reduced in a double mutant with bar-1(ga80) (Table 1)." With regard to vulval cell induction, pry-1(mu38) is epistatic to mig-14(ga62) and bar-1(ga80) is epistatic to pry-1(mu38).