In wild-type animals carrying either the cuIs2 or cuIs5 integrated reporter transgene, pharyngeal GFP expression is high because no nuclear DAF-3 activity is available to repress it. However, in TGF-b Daf-c mutants (e.g.
daf-7 or
daf-8), in which DAF-3 is de-repressed and active in the nucleus, GFP expression is greatly reduced.