pph-5 : sep-1

Double mutants were fully rescued from chromosome nondisjunction defects and cytokinesis failures during mitosis. Polar body extrusion was also fully rescued. Double mutant embryos were indistinguishable from wild type with regards to DAPI permeability and cortical granule (CG)quantification. The localization of SEP-1 to the meiotic spindle and cortical filaments was restored in addition to expression at the CG; however expression levels were reduced.

pph-5 : sep-1

Like av101, tm2979 behaves as a recessive suppressor of ax110. The localization of SEP-1 to the meiotic spindle and cortical filaments was restored in addition to expression at the CG; however expression levels were reduced similar to that seen with av101

unc-25 : unc-30

To determine the specificity of Pitx2 activation of unc-25/GAD, unc-30(e596) worms were co-injected with the Punc-30::Pitx2 expression con- struct along with either a wild-type Punc-25::GFP reporter or a mutant Punc-25::GFP reporter in which the UNC-30 binding sites were altered to CG-rich sequences. GFP expression was only detected from the wild type unc-25 reporter, supporting that Pitx2 transactivation is dependent on intact UNC-30 binding sites in the unc-25/GAD promoter.