"In two models of mitochondrial stress, feeding of spg-7(RNAi) as well as a previously characterized temperature-sensitive allele (zc32) (Benedetti et al., 2006), GFP fluorescence increased over the course of postembryonic development."
"In two models of mitochondrial stress, feeding of spg-7(RNAi) as well as a previously characterized temperature-sensitive allele (zc32) (Benedetti et al., 2006), GFP fluorescence increased over the course of postembryonic development."
"Worms were treated for 6 h on 51 mMNaCl agar plates containing either 1 mg/ml kasugamycin or 1 mg/ml cycloheximide. Kasugamycin inhibits translation initiation while cycloheximide blocks the elongation step of protein synthesis. As shown in Fig. 1E, both compounds elevated gpdh-1 mRNA levels 3-16 fold (P < 0.005)."
Treatment with BIBU1361 showed that the lower concentration 0.1 mg/ml induced beta-Galactosidase expression first in the intestinal cells of adult PC72 C. elegans. At higher concentrations (e.g., 0.4 mg/ml BIBU1361), a strong expression of h-Galactosidase was observed in cells of the head pharynx region and the midbody region.
"Worms were treated for 6 h on 51 mMNaCl agar plates containing either 1 mg/ml kasugamycin or 1 mg/ml cycloheximide. Kasugamycin inhibits translation initiation while cycloheximide blocks the elongation step of protein synthesis. As shown in Fig. 1E, both compounds elevated gpdh-1 mRNA levels 3-16 fold (P < 0.005)."