- transcription pausing by RNA polymerase II
A transcription halt following transcription initiation but prior to elongation, during which RNA polymerase II pauses approximately 20-60 nucleotides downstream of the transcriptional start site before proceeding into productive elongation. Transcription pausing starts following phosphorylation of the C-terminal domain (CTD) of RNA polymerase II subunit POLR2A at Ser-5 and stops following phosphorylation of POLR2A by the P-TEFb complex.
- DNA-templated transcription termination
The completion of transcription: the RNA polymerase pauses, the RNA-DNA hybrid dissociates, followed by the release of the RNA polymerase from its DNA template.
- replication fork arrest at tRNA locus
A process that impedes the progress of the DNA replication fork at natural replication fork pausing sites within the eukaryotic tRNA transcription unit.
- transcription elongation by RNA polymerase II
The extension of an RNA molecule after transcription pausing and promoter clearance at an RNA polymerase II promoter by the addition of ribonucleotides catalyzed by RNA polymerase II.
- NELF complex
A complex of five proteins, designated NELF-A, -B, -C, -D, and -E in human, that can physically associate with RNP polymerase II to induce transcriptional pausing.