PCK-2::YFP accumulated in the intestine, epidermis, body wall muscle, pharyngeal muscles, and sex muscles of both sexes; no neural expression was detectable. Expression of PCK-2 in the intestine and pharyngeal muscle is consistent with the earlier report that these tissues contain PEPCK activity (Yuan et al., 2016); however, in these tissues, the expression pattern suggests that the enzyme is likely expressed from
pck-2, instead of the
pck-1.