The egl-27 reporter is expressed in the Y cell in the 1.5-fold embryonic stage as well as during the L1 larval stage, which precedes the initiation of Y transdifferentiation.
TRA-2B:HA was detected exclusively in the nuclei of dissected tissues of larvae and adults, including those of the intestine and somatic gonad (sheath and distal tip cells). Nuclear localization of TRA-2B in somatic cells is consistent with previous studies using antibodies (Shimada et al., 2006) and reporter transgenes (Lum et al., 2000; Mapes et al., 2010)
wdr-5.1 was nuclear-localized in Y/PDA throughout transdifferentiation.In some cases faint additional WDR-5.1::GFP staining was observed in the cytoplasm.