We visualized mNG::LEA-1 fluorescence in dauer larvae. We found that the protein was prominently expressed in specific tissues: the germline, body wall muscle, pharynx, and excretory cell. mNG::LEA-1 was localized to both the excretory cell body, which is adjacent to the pharynx, as well as excretory canals, which extend laterally along the length of the body. Some faint expression was seen in seam cells (lateral hypodermal cells) , as well as the intestine. Additionally, some unidentified cells in the head were fluorescent.
sem-4 is expressed in all rectal cells in L1s hermaphrodites (i.e., Y, U, B, F, K, and K') and in all rectal cells, including P12.pa, in L3 or older worms.
About 20% GABAergic presynaptic terminals (RFP puncta) co-localized with postsynaptic NKB-1 (GFP) localization (103 co-localization with GFP puncta in totally observed 470 RFP puncta (n = 20 animals)). The majority of the postsynaptic Na+/K+ ATPase localization did not overlap with that of GABAergic synapses, suggesting a predominant localization of the Na+/K+ ATPase at cholinergic postsynaptic sites.
Pdaf-6GFP was expressed in the amphid sheath glia. Expression was also seen in amphid socket cells, the phasmid sensory organ sheath and socket cells, cells of the excretory system (the excretory canal, duct, pore, and gland cells), the vulval E and F cells, the K, K', F, and U rectal epithelial cells, and less frequently in posterior intestinal cells.