We obtained a cest-2.2p::GFP extrachromosomal array by microinjection and, consistent with previous evidence (Le et al, 2020), observed high GFP expression levels primarily in the intestine.
Using the ciliated neuron-specific labeling, we observed robust distribution of endogenous TBB-2 within amphid and phasmid ciliary MTs. Previous attempts using N- terminal GFP::TBB-2 KI allele had masked this phenomenon, likely due to background signals emanating from extraneous tissues. Furthermore, we found that ciliary TBB-2 was predominantly localized within the middle segments of cilia, with diminished signals in the distal segments, thereby revealing a unique and regulated distribution pattern distinct from that of TBB-4 distributing along the full-length cilia. These new findings were previously inaccessible with traditional labeling strategies. The hypodermis-specific labeling facilitated visualizing dynamic architecture of MT network in the hypodermal cell layers, effectively eliminating signal interference from adjacent tissues. The germline-specific labeling enabled tracking of the temporal behavior of mitotic and meiotic MTs within germline as well as in early-stage embryos, excluding background noise emanating from other unrelated tissues.