Expr13289

prkl-1 is expressed in VNC neurons. In contrast to Prickle localization in other CE models (Jiang et al., 2005; Narimatsu et al., 2009; Yin et al., 2008), PRKL-1 (n = 6) was not observed to exhibit recognizable membrane or polarized subcellular localization. Relatively uniform PRKL-1 cytoplasmic localization was observed in VNC neurons.

Expr3842

daf-18 message was present predominantly in the germline in larvae and adults (93%, n = 88). This is a specific signal not detected in the daf-18 deletion mutant (n = 89) or in glp-4(bn2) animals (n = 112), where little germline proliferation occurs.

Expr4229

Pegl-1gfp is expressed in 0% of the HSNs in wild-type XX animals (n = 27).

Expr8936

UNC-17 antibody staining in AIA can be observed in 94% of wild-type animals (n=32).

Expr16121

We did not observe expression of endogenously tagged DLG-1::mNG protein in larval AQR (n 1⁄4 30).

Expr14679

A fosmid transgene containing sax-7p::TY1::EGFP was expressed in the DTC and localized to the membrane (n = 7/7).

Expr9069

DRAG-1::GFP was present in pharyngeal, hypodermal and intestinal cells. Robust expression in the M lineage was also detected from the 1-M to 4-M stage. DRAG-1::GFP signal was reduced (40%, n=20) or undetectable (60%, n=20) at the 8-M stage, and completely undetectable beyond the 8-M stage.

Expr8940

Expressed by the male linker cell from the early L4 stage until LC death in the L4-to-adult molt (n=40/41)

Expr13399

In support of a role of ARX-2 in Q cell corpse clearance, ARX-2::GFP was recruited onto Q cell corpses at 37 ± 9 min (mean ± SD; n = 20) after Q cell birth, and the ARX-2::GFP rings lasted for 19 ± 9 min (n = 20). The corpse was completely degraded 34 ± 18 min (n = 20) after the disappearance of ARX-2::GFP, which suggests that ARX-2 may function during the engulfment of Q cell corpses but may not be involved in the final degradation inside the phagosome.

Expr15953

mNG::mls-2 knock-in animals displayed wild-type AWC asymmetry as determined by the expression of the AWCON marker str-2p::TagRFP, suggesting that mNG::MLS-2 fusion protein is functional in AWC development. Like GFP::MLS-2 expressed from transgenes, mNG::MLS-2 knock-in was localized in the nucleus of AWC precursor cells in early embryos but was not observed in AWC cells in late embryos or early-stage larvae. Similar to MLS-2 antibody staining and GFP::MLS-2 transgenes (Jiang et al., 2005), mNG::MLS-2 knock-in was localized to the nucleus of a subset of head cells and the M mesoblast in first-stage larvae and adults.