sem-4 is expressed in all rectal cells in L1s hermaphrodites (i.e., Y, U, B, F, K, and K') and in all rectal cells, including P12.pa, in L3 or older worms.
About 20% GABAergic presynaptic terminals (RFP puncta) co-localized with postsynaptic NKB-1 (GFP) localization (103 co-localization with GFP puncta in totally observed 470 RFP puncta (n = 20 animals)). The majority of the postsynaptic Na+/K+ ATPase localization did not overlap with that of GABAergic synapses, suggesting a predominant localization of the Na+/K+ ATPase at cholinergic postsynaptic sites.