Expr13483

Expression of mapk- 15 isoform A-fused to gfp and C-fused to mCherry was observed in non-dye-filling (ASE, BAG, CEP, IL1, IL2, and OLQ) and dye-filling (ADL, ADF, ASH, ASI, ASJ, and ASK) head CSNs, as well as in nondye filling (PQR) and dye filling (PHA, PHB) tail CSNs. Expression was also occasionally observed in mid-body neurons (PDE).

Expr13484

Expression of mapk- 15 isoform A-fused to gfp and C-fused to mCherry was observed in non-dye-filling (ASE, BAG, CEP, IL1, IL2, and OLQ) and dye-filling (ADL, ADF, ASH, ASI, ASJ, and ASK) head CSNs, as well as in nondye filling (PQR) and dye filling (PHA, PHB) tail CSNs. Expression was also occasionally observed in mid-body neurons (PDE).

Expr11158

ncx-4 is expressed in AWC, ASE, two pair of labial neurons, ventral/dorsal nerve cord and commissures, faint expression in one pair of non-dye filling posterior neurons.

Expr14984

We confirmed that daf-11 is expressed in seven pairs of head sensory neurons: dye-filling (DiI) positive ASK, ADL, ASI, AWB, ASH, ASJ neurons and Dil negative AWC olfactory neurons.

Expr10712

mct-1 is expressed in the pharynx. The expression pattern persisted from hatching to adulthood, and GFP-tagged MCT-1 was not detected in six amphid sensory neurons that are labeled by DiI dye.

Expr8322

The punctuate fluorescent signals were observed in the cell bodies of the head and tail neurons. These signals were detected in all of the dye-filling assay positive cells, suggesting that IFT-81 is expressed in ADL, ASH, ASI, ASJ, ASK, AWB, PHA, PHB ciliary sensory neurons.

Expr8603

DAF-6::GFP was expressed in the sheath and socket cells, as well as in the excretory canal at all stages of animals. In dauers, DAF-6::GFP expression in these cells was dramatically reduced/eliminated, although a pair of small puncta at the tip of the sheath cells remained in some animals, and most of the animals failed dye filling. At the same time, DAF-6::GFP colocalized with a dauer-specific cuticular structure called dauer alae. DAF-6::GFP expression in the sheath and socket cells was fully restored within 24 h when dauers were allowed to recover under optimal growth conditions, coincident with recovery of dye filling.

Expr12108

A transcriptional reporter expressing GFP driven by the OSG-1 promoter, (Posg-1::GFP, FDX2533 strain), yielded signals in body wall muscle, head muscle and in several neurons in the head including FLP neurons. Posg-1::GFP expression was also detected in the ASE neurons whose identification was confirmed by DiD dye staining.

Expr11567

Confocal laser microscopy revealed strong OXY-5::GFP fluorescence in neurons in the head and tail, in the pharynx, and in the intestine. The expression of OXY-5 in neurons was confirmed by co-localization of several signals of OXY-5::GFP with those of chemosensory neurons stained with a fluorescent dye DiQ.

Expr16221

A translational acd-5::mKate2 C-terminal fusion showed expression in the intestinal epithelial cells, localising to the apical membrane, as confirmed by co-localisation with an apical membrane marker (OPT-2::GFP [Nehrke, 2003]) and by comparing the localisation with that of markers for the intestinal cytoplasm (Pdel-5::GFP) and lumen (ingested fluorescent dye, DiO.