Expr2771

GFP-tagged synaptojanin was localized to puncta at neuromuscular junctions in the ventral nerve cord of unc-26 animals (genotype oxIs92 II; unc-26(s1710) IV).

Expr14702

Consistent with previous studies, the type-IV collagen α1 chain construct, EMB-9::mCherry, when expressed from the integrated transgene tkTi1[emb-9::mCherry], localized to the gonadal basement membrane in WT C. elegans.

Expr14507

Type IV collagen localizes to the BM and is expressed primarily in the body wall muscles, with the neuronal ring ganglion, spermatheca, uterine mucles, vulval muscles, and the distal tip cell of the somatic gonad also contributing collagen during specific larval stages.

Expr1420

A gradual increase in the expression level of CPTA during the development from embryo through larva L1-2 and L3-4 to adults was clearly evident. The relative intensities of the CPTA-specific product with respect to actin IV were 2.7, 2.7, 7.0 and 7.8 for the eggs, L1-2, L3-4 and mixed stage animals respectively.

Expr15292

As expected, localization of mkate2::HIM-4 to the pharyngeal basement membrane was observed at the L1 stage. In contrast to the uniform localization on the basement membrane of type IV collagen or nidogen, which are major components of the basement membrane, that of mkate2::HIM-4 was polarized at the anterior part of the pharyngeal basement membrane. A unique polarized localization pattern was also observed in the pharyngeal basement membrane at stage L4. These observations indicate that polarized HIM-4 localization in the anterior part of the pharyngeal BM is maintained throughout the L1-L4 stages. The localization was also observed at the rachis during gonadal development at the L4 stage; this observation was consistent with that of previous reports.

Expr16562

We first used a previously characterized dmd-3::YFP transcriptional reporter that contains 4264 nucleotides upstream of the start codon, the first exon, the first intron and part of the second exon fused to YFP (Mason et al., 2008) to examine the dynamics of dmd-3 expression in the linker cell and hindgut in L1 - L4 males. We found that dmd-3: YFP expression initiates in the linker cell in L3 males when the linker cell is midway through stage II and persists until linker cell engulfment and death. In addition, we observed that dmd-3::YFP is expressed in a subset of posterior presumptive vas deferens cells that contact the trailing side of the migrating linker cell. Expression in the vas deferens initiates during or closely following the turning of the linker cell to the ventral side (mid-stage III) and persists as the linker cell migrates to the hindgut (stage IV). dmd-3::YFP expression in the hindgut cells of the U lineage commences in L1/L2 males, coincident with stage I of linker cell migration, and persists through linker cell engulfment and hindgut morphogenesis in late L4 males. We do not observe dmd-3::YFP expression in the distal tip or hindgut cells of L4 hermaphrodites (Mason et al., 2008).