WBAntibody00002705

Peptide-derived polyclonal antibodies were generated in rabbits against subunit UNC-29.1 (21st Century Biochemicals-Marlborough, MA). Animals were immunized with a mixture of two specific peptides corresponding to parts of the intracellular loop located between the third and the fourth TM domains. For UNC-29.1, the synthetic peptides were 'EKTASPKTLNCAMELTTRDPQL' and 'PIPEPAPPEITQLP'. Purified antibodies were tested for specificity and titrated by ELISA. unc-29

WBAntibody00002706

Peptide-derived polyclonal antibodies were generated against subunits UNC-29.2 in rats (21st Century Biochemicals-Marlborough, MA). Animals were immunized with a mixture of two specific peptides corresponding to parts of the intracellular loop located between the third and the fourth TM domains. For UNC-29.2, the synthetic peptides were 'VSIKVLNCGDDIPKDP' and 'MTKKKRGATVAKLP'. Purified antibodies were tested for specificity and titrated by ELISA. unc-29

WBAntibody00002958

To obtain specific antibodies against T09F3.2 and FGT-1, we selected three peptides, CPLEVVKTRMQSSRGLDA (peptide A cor- responding to T09F3.2), CHEVVRTRLREETGKSRG (peptide B corresponding to T09F3.2), and CSALKKLRDTEDVSTEIEA (peptide C corresponding to FGT-1). Antibodies against these peptides (poly- clonal, rabbit) were purchased from Sigma-Aldrich, Japan. Anti- bodies were affinity-purified using SulfoLink® Coupling Resin (Thermo Fisher Scientific, MA, USA) according to the manufac- turer's instructions. fdgt-1

WBAntibody00002957

To obtain specific antibodies against T09F3.2 and FGT-1, we selected three peptides, CPLEVVKTRMQSSRGLDA (peptide A cor- responding to T09F3.2), CHEVVRTRLREETGKSRG (peptide B corresponding to T09F3.2), and CSALKKLRDTEDVSTEIEA (peptide C corresponding to FGT-1). Antibodies against these peptides (poly- clonal, rabbit) were purchased from Sigma-Aldrich, Japan. Anti- bodies were affinity-purified using SulfoLink® Coupling Resin (Thermo Fisher Scientific, MA, USA) according to the manufac- turer's instructions. T09F3.2

WBAntibody00002762

To produce antibodies against the NES like sequence of SOD1, a SOD1 peptide corresponding to residues 33-51 (GSIKGLTEGLHGFHVHEFGC) was synthesized by Peptide 2.0 Inc. (Chantilly, VA). A Cysteine was added to the C-terminus for conjugation of the peptide to keyhole limpet hemocyanin (KLH) to improve the immunogenicity. Anti-NLP was purified from rabbit antiserum using the same peptide that was biotinylated at the C-terminus and bound to streptavidin magnetic beads (Thermo scientific, Waltham, MA). sod-1