8 Antibodies found (1.152 seconds)

Monoclonal antibody against UNC-54, from H. F. Epstein (UTMB, Gavleston, TX). unc-54

Peptide-derived polyclonal antibodies were generated in rabbits against subunit UNC-29.1 (21st Century Biochemicals-Marlborough, MA). Animals were immunized with a mixture of two specific peptides corresponding to parts of the intracellular loop located between the third and the fourth TM domains. For UNC-29.1, the synthetic peptides were 'EKTASPKTLNCAMELTTRDPQL' and 'PIPEPAPPEITQLP'. Purified antibodies were tested for specificity and titrated by ELISA. unc-29

WBAntibody00002706

Peptide-derived polyclonal antibodies were generated against subunits UNC-29.2 in rats (21st Century Biochemicals-Marlborough, MA). Animals were immunized with a mixture of two specific peptides corresponding to parts of the intracellular loop located between the third and the fourth TM domains. For UNC-29.2, the synthetic peptides were 'VSIKVLNCGDDIPKDP' and 'MTKKKRGATVAKLP'. Purified antibodies were tested for specificity and titrated by ELISA. unc-29

WBAntibody00003123

Blots were incubated with primary antibody for 1.5 h at room temperature (anti-HSP-6, a kind gift from the Morimoto lab) hsp-6

WBAntibody00002958

To obtain specific antibodies against T09F3.2 and FGT-1, we selected three peptides, CPLEVVKTRMQSSRGLDA (peptide A cor- responding to T09F3.2), CHEVVRTRLREETGKSRG (peptide B corresponding to T09F3.2), and CSALKKLRDTEDVSTEIEA (peptide C corresponding to FGT-1). Antibodies against these peptides (poly- clonal, rabbit) were purchased from Sigma-Aldrich, Japan. Anti- bodies were affinity-purified using SulfoLink® Coupling Resin (Thermo Fisher Scientific, MA, USA) according to the manufac- turer's instructions. fdgt-1

WBAntibody00002957

WBAntibody00002762

To produce antibodies against the NES like sequence of SOD1, a SOD1 peptide corresponding to residues 33-51 (GSIKGLTEGLHGFHVHEFGC) was synthesized by Peptide 2.0 Inc. (Chantilly, VA). A Cysteine was added to the C-terminus for conjugation of the peptide to keyhole limpet hemocyanin (KLH) to improve the immunogenicity. Anti-NLP was purified from rabbit antiserum using the same peptide that was biotinylated at the C-terminus and bound to streptavidin magnetic beads (Thermo scientific, Waltham, MA). sod-1

WBAntibody00002768

The N-terminal portion (aa 4-77) of CYB-1 was cloned into a maltose-binding protein (MBP)-tagged 6xHis vector, pYS, as follows. First, the MBP gene was PCR-amplified from pHIT198 (a gift from H. Tabara, Tsukuba University, Kyoto, Japan) and cloned into pET- 28a(+) (Novagen, 69864-3) by means of the DNA Ligation Kit Mighty Mix (Takara, 6023) to generate pYS. Second, the N-terminal portion (aa 4-77) of CYB-1 was amplified from yk1306b09 (a gift from Y. Kohara, National Institute of Genetics, Mishima, Japan) and cloned into the pYS vector. The CYB-1-MBP-6xHis fusion protein was expressed and affinity-purified using His-Bind Agarose Resin (ELPIS-BIOTECH, EBE-1031). It was then injected into rats as an antigen to raise an anti-CYB-1 antibody (AbFrontier). This antibody was then affinity-purified from the crude serum, using 6xHis-tagged CYB-1-GST. The GST gene was amplified from pDEST15 (Invitrogen, 11802-014) and inserted into pET-28a(+) (Novagen, 69864-3) to generate pYS1. The same N-terminal portion of CYB-1 used to produce the CYB-1-MBP-6xHis fusion protein was cloned into the pYS1 vector. cyb-1