WBAntibody00002816

A GST fusion protein of LMW-PTP (GST-LMW-PTP; Y94H6A.7) was purified from an E. coli expression system harboring the pGex-2T vector. The pu- rified protein was used to immunize a rabbit to generate anti-serum. The antibody was then purified through negative selection on GST-aga- rose and subsequently positive selection on GST-LMW-PTP-agarose. Y94H6A.7

WBAntibody00003109

Sequences encoding full-length EDC-3, CGH-1 and a 37 kDa fragments of EDC-4 (sequence: NAEDSGDDLF-(314 amino acids)- QKCLVSLVSQ) were individually cloned into pSMT3 plasmid (N-terminal 6XHis-SUMO). Each construct was expressed in and pu- rified from BL21(DE3) cells. Two mg of proteins were used in four rounds of rabbit injection (Capralogics, Cambridge Massachusetts) and a total of three bleeds were collected and characterized. cgh-1

WBAntibody00003111

Sequences encoding full-length EDC-3, CGH-1 and a 37 kDa fragments of EDC-4 (sequence: NAEDSGDDLF-(314 amino acids)- QKCLVSLVSQ) were individually cloned into pSMT3 plasmid (N-terminal 6XHis-SUMO). Each construct was expressed in and pu- rified from BL21(DE3) cells. Two mg of proteins were used in four rounds of rabbit injection (Capralogics, Cambridge Massachusetts) and a total of three bleeds were collected and characterized. edc-4

WBAntibody00003110

Sequences encoding full-length EDC-3, CGH-1 and a 37 kDa fragments of EDC-4 (sequence: NAEDSGDDLF-(314 amino acids)- QKCLVSLVSQ) were individually cloned into pSMT3 plasmid (N-terminal 6XHis-SUMO). Each construct was expressed in and pu- rified from BL21(DE3) cells. Two mg of proteins were used in four rounds of rabbit injection (Capralogics, Cambridge Massachusetts) and a total of three bleeds were collected and characterized. edc-3