MACSPI Enables Tissue-Selective Proteomic and Interactomic Analyses in Multicellular Organisms

To define the proteome and interactome of a specific type of tissue in an intact animal, authors developed a localized proteomics approach called Methionine Analog-based Cell-Specific Proteomics and Interactomics (MACSPI). This method uses the tissue-specific expression of an engineered methionyl-tRNA synthetase (MetRS) to label proteins with a bifunctional amino acid photo-ANA in selected cells. Authors applied MACSPI in Caenorhabditis elegans, a model multicellular organism, to selectively label, capture, and profile the proteomes of the body wall muscle and the nervous system, which led to the identification of new tissue-specific proteins.

MACSPI Enables Tissue-Selective Proteomic and Interactomic Analyses in Multicellular Organisms

To define the proteome and interactome of a specific type of tissue in an intact animal, authors developed a localized proteomics approach called Methionine Analog-based Cell-Specific Proteomics and Interactomics (MACSPI). This method uses the tissue-specific expression of an engineered methionyl-tRNA synthetase (MetRS) to label proteins with a bifunctional amino acid photo-ANA in selected cells. Authors applied MACSPI in Caenorhabditis elegans, a model multicellular organism, to selectively label, capture, and profile the proteomes of the body wall muscle and the nervous system, which led to the identification of new tissue-specific proteins.

MACSPI Enables Tissue-Selective Proteomic and Interactomic Analyses in Multicellular Organisms

To define the proteome and interactome of a specific type of tissue in an intact animal, authors developed a localized proteomics approach called Methionine Analog-based Cell-Specific Proteomics and Interactomics (MACSPI). This method uses the tissue-specific expression of an engineered methionyl-tRNA synthetase (MetRS) to label proteins with a bifunctional amino acid photo-ANA in selected cells. Authors applied MACSPI in Caenorhabditis elegans, a model multicellular organism, to selectively label, capture, and profile the proteomes of the body wall muscle and the nervous system, which led to the identification of new tissue-specific proteins.

MACSPI Enables Tissue-Selective Proteomic and Interactomic Analyses in Multicellular Organisms

To define the proteome and interactome of a specific type of tissue in an intact animal, authors developed a localized proteomics approach called Methionine Analog-based Cell-Specific Proteomics and Interactomics (MACSPI). This method uses the tissue-specific expression of an engineered methionyl-tRNA synthetase (MetRS) to label proteins with a bifunctional amino acid photo-ANA in selected cells. Authors applied MACSPI in Caenorhabditis elegans, a model multicellular organism, to selectively label, capture, and profile the proteomes of the body wall muscle and the nervous system, which led to the identification of new tissue-specific proteins.